Effect of Picrorhiza kurroa Royle ex Benth ethanolic root extract on Cardio protection against methotrexate induced myocardial infarction in rats

Author: Ramalakshmi Y.V.A., Manibalan V. and Manivannan E.

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Abstract

The purpose of this research was to examine whether Picrorhiza kurroa Royle ex Benth (ERPK) root extracts could prevent Methotrexate (MTX)-induced heart damage in rats. Methotrexate (20 mg/100g, s.c.) was administered twice at 24-hour intervals to the animals. Analysis of electrocardiogram (ECG) parameters, serum marker enzymes, and cardiac histology were used to determine cardio-protective efficacy. P wave, QRS complex, and R-R interval all increased significantly (P< 0.001) after pretreatment with 200 and 400mg/kg of ERPK, whereas heart rate, QT interval, and cardiac cycle remained within acceptable ranges. All of the diagnostic indicators assessed for ERPK were significantly (P <0.05; P< 0.001) lower in that group than in the MTX-treated group. The protective function of ERPK was substantiated by histological examinations of cardiac tissue alterations. These findings imply that ERPK therapy before MTX plays a crucial role in preventing MTX-induced myocardial infarction in rats.

Keywords

Picrorhiza kurroa, Methotrexate, ECG, Marker enzyme, Cardioprotective

Conclusion

The present investigation demonstrated that fractions of the root of Picrorhiza kurroa protected myocardium from the structural and functional damage caused by Methotrexate. ERPK was shown to include flavonoids, tannins, and phenolics in the phytochemical analyses. The existence of these bioactive components and their synergistic capabilities may account for the cardio-protective activity of ERPK. The cardio-protective activity may also occur because beta-adrenergic receptor stimulation is inhibited, which reduces the production of reactive oxygen species and keeps the myocardial membranes healthy. The present study's findings support the use of Picrorhiza kurroa as a cardio-protective agent by showing that its extracts restored normal levels of electrophysiological, biochemical, and histopathological parameters in experimental rats given Methotrexate.

References

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How to cite this article

Ramalakshmi Y.V.A., Manibalan V. and Manivannan E. (2023). Effect of Picrorhiza kurroa Royle ex Benth ethanolic root extract on Cardio protection against methotrexate induced myocardial infarction in rats. Biological Forum – An International Journal, 15(6): 781-786.