Evaluation of Fungicide compatibility with a Co-culture of Trichoderma spp. under in vitro Conditions

Author: A.G. Panda, B. Boblina, M.K. Mishra, A.K. Senapati, D. Datta and B. Jena

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Abstract

For agriculture to be sustainable, soil-borne plant diseases must be effectively controlled. The compatibility of two native Trichoderma spp. strains (TAKJR and THPUR) with six fungicides to combat Rhizoctonia solani is examined in this study. We assessed the inhibitory effects of fungicides at doses between 100 PPM and 1500 PPM. Hexaconazole mildly inhibited TAKJR (46.7%), according to the results, but at 1500 PPM, the inhibition dramatically increased to 93.3%. At 1500 PPM, Difenoconazole demonstrated substantial (95.6%) inhibition of TAKJR. At 1500 PPM, Tebuconazole showed moderate inhibition, rising to 90.6%. At 100 PPM, inhibition by Propineb increased to 48.6% at 1500 PPM. Moderate inhibition was seen with Propiconazole. Pencycuron, the most suitable fungicide, showed little inhibition at 1500 PPM (11.9%). Hexaconazole and Difenoconazole had excellent inhibitions at 1500 PPM (96.7% and 95.6%, respectively), while THPUR demonstrated similar responses. The inhibition of Tebuconazole ranged from 40.8% to 90.6%. Pencycuron and Propineb demonstrated negligible inhibitory effects. The study shows that fungicides have inhibitory effects on Trichoderma spp. in a dose-dependent manner. Their inclusion in an integrated disease control schedule is suggested by their compatibility with particular fungicides, most notably Pencycuron.

Keywords

Trichoderma, Fungicide compatibility, Pencycuron

Conclusion

According to the compatibility test, various fungicides, including Hexaconazole and Difenoconazole, significantly inhibited Trichoderma at all doses examined. Propineb and Pencycuron, on the other hand, showed no inhibition even at greater doses, indicating compatibility with Trichoderma. Tebuconazole and propiconazole showed only modest inhibition, with higher doses having a greater effect. When developing sustainable plant disease control techniques, the information from this research study emphasizes the need of carefully considering how fungicides affect beneficial organisms like Trichoderma. Although Hexaconazole and Difenoconazole have strong antifungal properties, they may also have a negative impact on populations of Trichoderma. Tebuconazole, Propineb, and Pencycuron, on the other hand, show increased compatibility with Trichoderma, making them suitable candidates for integrated pest management programs meant to take use of this helpful fungus' biocontrol capabilities. In the end, our findings offer insightful information for fungicide-Trichoderma combination optimization, providing efficient disease control while safeguarding the critical ecological functions of Trichoderma in agricultural settings. Moving forward, these findings present a promising avenue for advancing sustainable plant disease management. The study's emphasis on understanding fungicide effects on beneficial organisms like Trichoderma underscores the importance of incorporating compatibility assessments into agricultural practices. Further investigation into the mechanisms underlying the variable inhibition patterns, as well as field validation of the compatibility of Tebuconazole, Propineb, and Pencycuron with Trichoderma, can refine integrated pest management strategies. This research sets the stage for optimizing fungicide-Trichoderma combinations to achieve effective disease control while upholding pivotal ecological roles of Trichoderma within agricultural ecosystems.

References

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How to cite this article

A.G. Panda, B. Boblina, M.K. Mishra, A.K. Senapati, D. Datta and B. Jena (2023). Evaluation of Fungicide compatibility with a Co-culture of Trichoderma spp. under in vitro Conditions. Biological Forum – An International Journal, 15(1): 685-688.