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A First Report on Buttermilk Isolate Dipodascus armillariae and its Improved Strain DAM 5 as a Novel Probiotic

Author: Vijayalakshmi S., Anagha J. and Keerthana B.

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Abstract

A new era in the probiotic family is emerging with the identification of many fungal strains as probiotics. Fungi are excellent candidates for probiotic flora due to their distinct cellular structures and enhanced capacity to survive in the hostile environment of the gastrointestinal system. Here, probiotics formulated using buttermilk isolate of Dipodascus armillaria and its caffeine improved variant (DAM 5) were compared with conventional strain of Lactobacillus acidophilus. Probiotic characterization of isolates revealed pH 1 tolerance, high antimicrobial activity and increased lactic acid production by DAM5. In this endeavour, an effort was made to create nutritionally enhanced probiotic drink, using the medicinal plant extracts of Alternanthera sessilis & Eclipta prostrata, with the chosen strains and assessed for their capacity to endure in or ferment these plants' leaf extracts. Two weeks fermented plant extracts revealed improving acidity, low pH, higher turbidity, antimicrobial & antioxidant activity and an increase in energy content of the chosen isolates. A. sessilis proved to be a best plant substrate compared to E. prostrata. Statistical analysis revealed the use of DAM 5 to be significant in probiotic formulation compared to other isolates. Extended study improving their shelf life can be better future prospect.

Keywords

Probiotics, Alternanthera sessilis, Eclipta prostrata, Dipodascus armillaria, DAM 5

Conclusion

In the present study entitled an attempt has been made to isolate an efficient potential probiotic yeast strain from buttermilk, its improvement with caffeine and further evaluation of their essential probiotic properties. Presence of Dipodascus armillariae in buttermilk has been revealed and its utilization as a probiotic becomes novel and significant as the results concluded higher pH tolerance (at pH 1), better antimicrobial efficacy (23mm) and enhanced lactic acid production (0.6%). Use of caffeine being a natural stimulant effective in probiotic enhancement is also proven as 1mM concentration showed 16% increase in lactic acid production in 24 hours of study. Hence, due to these properties this particular improved variety Dipodascus armillaria treated with 1mM caffeine (namely, DAM 5) was identified to be used in future for probiotic product formulations.

References

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How to cite this article

Vijayalakshmi S., Anagha J. and Keerthana B. (2023). A First Report on Buttermilk Isolate Dipodascus armillariae and its Improved Strain DAM 5 as a Novel Probiotic. Biological Forum – An International Journal, 15(5a): 527-531.