Protective Effect of Curcumin and Ascorbic acid Against Lead acetate Induced Cardiotoxicopathology in Wistar Rats

Author: Karthik I., Dubey A., Swamy M., Tiwari A., Verma Y., Naveen D. and Rakesh S.

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Abstract

Lead is a potential toxic heavy metal that exhibits deteriorating effects on various organ systems of body. Accordingly, the present study was aimed to investigate the ameliorative effect of of curcumin and ascorbic acid against lead induced cardiotoxicopathology in Wistar rats. Thirty-two Wistar rats of 6-8 weeks of age were randomly divided into four groups with eight rats each, as (Group-I) control group, (Group-II) (150 mg/kg b.wt. lead acetate), (Group-III) (150 mg/kg b.wt. lead-acetate, 400 mg/kg b.wt. curcumin and 420 mg/kg b.wt. ascorbic acid) and Group-IV (400 mg/kg b.wt. curcumin and 420 mg/kg b.wt. ascorbic acid). All the experimental groups received oral treatments for 30 consecutive days. Significant increase in blood lead level, serum AST, ALT, CKMB, cardiac troponin I (cTnI) and cardiac tissue MDA level was observed in group II rats. Significant histopathological findings (myocardial necrosis, vacuolation, fibrosis and loss of cross striations) were observed in group II rats. Administration of curcumin and ascorbic acid against lead acetate in group III rats showed significant amelioration in lead induced cardiotoxicity via decrease in cardiac troponin I and cardiac tissue MDA level along with significant improvement in histo-architecture of heart. Thus, the present study revealed that lead exposure has toxic effects on heart which disturb its functioning, while natural these antioxidants may be preferable in reducing lead induced cardiotoxicity suggesting that chelating agents having antioxidant properties are preferred in treating cardiovascular disorders accompanying lead toxicity.

Keywords

Cardiotoxicity, Lead acetate, Curcumin, Ascorbic acid, ECG, Histopathology

Conclusion

In conclusion, the present investigation enunciated that lead acetate induced the significant cardiotoxicity due to the excess generation of free radicals and impairment of antioxidant defences that subsequently resulted in significant alterations in several parameters pertaining to heart. Use of curcumin and ascorbic acid countered the adverse effects of lead induced cardiotoxicity to a major extent suggesting its antioxidant potential owing to depletion of tissue pool of MDA. Also, this study suggested that lead chelating agents having antioxidant properties are preferred in treating cardiovascular disorders accompanying lead toxicity.

References

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How to cite this article

Karthik I., Dubey A., Swamy M., Tiwari A., Verma Y., Naveen D. and Rakesh S. (2023). Protective Effect of Curcumin and Ascorbic acid Against Lead acetate Induced Cardiotoxicopathology in Wistar Rats. Biological Forum – An International Journal, 15(9): 114-122.