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Antimicrobial Analysis of Centella asiatica L. and Hydrocotyle verticillata Thunb.

Author: S.M. Dhivya, P. Vijayashalini, V. Sasmitha and M. Dhivyasree

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Abstract

Microorganisms that were both susceptible to and resistant to antibiotics were used to assess the antibacterial activity of plant extracts and phytochemicals. The failure of the equipment, temperature, moisture, and antimicrobial agent potency are only a few of the variables that can have an impact on the outcomes of an antibiotic sensitivity test. The purpose of this research is to investigate the antimicrobial efficacy in Centella asiatica and Hydrocotyle verticillata against the gram positive (Staphylococcus aureus, Bacillus subtilis) and gram negative (Escherichia coli, Klebsiella pneumoniae) bacteria. The study revealed that the benzene and ethanol extracts of both Centella asiatica and Hydrocotyle verticillata inhibited the growth of all the tested bacteria. The benzene extract of Centella asiatica inhibits the bacteria Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Klebsiella pneumoniae with a zone of inhibition of 9.7 ± 0.2mm, 8.6 ± 0.3mm, 7.8 ± 0.2mm and 6.2 ± 0.3mm, respectively and the same extract of Hydrocotyle verticillata showed an inhibition zone of 10.6 ± 0.2mm, 9.6 ± 0.3mm, 8.2 ± 0.2mm and 7.4 ± 0.3mm respectively. In the same way, the ethanol extract of Centella asiatica inhibits the bacteria Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Klebsiella pneumoniae with a zone of inhibition of 11.1 ± 0.3mm, 10.0 ± 0.1mm, 8.2 ± 0.1mm and 6.4 ± 0.2mm respectively and the ethanol extract of Hydrocotyle verticillata showed an inhibition zone of 12.4 ± 0.3mm, 10.2 ± 0.1mm, 8.4 ± 0.1mm and 6.8 ± 0.2mm respectively. The results concluded that the study plants have effective antimicrobial properties.

Keywords

Antimicrobial, Staphylococcus aureus, inhibition and Ethanol

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How to cite this article

S.M. Dhivya, P. Vijayashalini, V. Sasmitha and M. Dhivyasree (2023). Antimicrobial Analysis of Centella asiatica L. and Hydrocotyle verticillata Thunb. Biological Forum – An International Journal, 15(5a): 553-557.