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Anterior Pituitary Gland Prolactin Expression Profile of IWI Layer and Kadaknath Chicken during 20 and 40 Weeks of Age

Author: B. Rajith Reddy, B. Ekambaram and T.K. Bhattacharya

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Abstract

A study was conducted to explore mRNA expression profiles of the prolactin gene in the anterior pituitary glands in two varieties of chicken viz., IWI layer and Kadaknath birds both at 20 and 40 weeks of age. In the present experiment, the anterior pituitary glands of three birds from each breed were collected and their total RNA was extracted, and further proceeded for cDNA synthesis. The RT-qPCR study revealed that Kadaknath chicken during 40 weeks had the highest prolactin expression, while the lowest prolactin was observed at 20 weeks in the IWI layer among all the groups. Further, the rise in prolactin expression was minimal in the IWI layer between 20 and 40 weeks with a fold change rise of 1 to 1.35. However, the rise of fold change from 1 to 3.5 at 20 and 40 weeks was observed in Kadaknath chicken which indicates that the rise in prolactin expression is high compared to the IWI layer. It has been concluded that the prolactin expression levels in the pituitary gland are higher in the Kadaknath breed than in the IWI layer, and it increases as age progresses in Kadaknath chickens. According to this expression profile, changes in prolactin levels at different ages may be the reason of high egg production in layers compare to native chickens with lower egg production.

Keywords

IWI layer, Kadaknath, Prolactin, RT-qPCR, Expression

Conclusion

The present investigation revealed low levels of prolactin expression inlayers compared to kadaknath chicken and the increment of prolactin is more in kadaknath chicken than IWI layers as age advances.

References

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How to cite this article

B. Rajith Reddy, B. Ekambaram and T.K. Bhattacharya (2023). Anterior Pituitary Gland Prolactin Expression Profile of IWI Layer and Kadaknath Chicken during 20 and 40 Weeks of Age. Biological Forum – An International Journal, 15(9): 258-262.