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Cultural and Morphological Characterization of Ralstonia solanacearum causing Chilli Bacterial Wilt

Author: Abhisek Tripathy, Akshaya Kumar Senapati and Satya Narayan Satapathy

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Abstract

In the coastal vegetable belt of Odisha, Ralstonia solanacearum-caused chilli bacterial wilt is a fairly well-known disease. It causes around 100 percent yield loss in the farmer’s field. The condition is extremely complex, and numerous physical, cultural, chemical, and biological approaches have been tried and failed to control it. The purpose of this study is to determine the diversity of the various isolates and their chances of survival. Five isolates were obtained for this study from the Odisha coastal vegetable region. RS 1, RS 2, RS 3, RS 4, and RS 5 were the names given to the various isolates. They were also examined for diversity studies, including cultural and physical differences. They were all evaluated for colony growth in various growth medium, and it was discovered that TZC media and CPG media were the best. Ralstonia solanacearum's cultural traits, including colony color, colony count, and colony form, were examined in vitro using the four-culture media. The TZC agar plate had the highest average colony count (72.00), which featured white fluidal colonies with pink spherical centres, and the lowest average colony count (56.00), which featured irregular yellow colonies. From this study, we got an idea about the diversity among various isolates of Ralstonia which in turn will help to manage this particular problem efficiently.

Keywords

Ralstonia solanacearum, culture media, Chilli, Triphenyl tetrazolium chloride, Nutrient Agar, Mac conkey agar, CPG agar

Conclusion

According to the above test results, TZC media is the best media for the growth of R. solanacearum among the other media. To justify it TZC media showed highest amount of growth of the bacteria i.e. 72.00.

References

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How to cite this article

Abhisek Tripathy, Akshaya Kumar Senapati and Satya Narayan Satapathy (2023). Cultural and Morphological characterization of Ralstonia solanacearum causing Chilli Bacterial Wilt. Biological Forum – An International Journal, 15(1): 689-693.