Application of Micronucleus Assay for Assessment of Profenofos Genotoxicity and its Mitigation through Nyctanthes arbortristis Leaf Extract in Mice (Mus musculus)

Author: Rina Kumari and Dharmshila Kumari

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Abstract

The micronucleus test is always included in many genotoxic test guidelines for long time in many classes of chemicals, e.g., pharmaceutical chemicals, agricultural chemicals, food additives. The most commonly used agrochemical profenofos has adverse effect among human population. The dependence of people on the pesticides have may effect various organ system like respiratory system, reproductive system, etc. and also leading to genetic toxicity. In present work the mitigative effect of Nyctanthes arbortristis leaves were evaluated against genotoxic damage induced by Profenofos on PCEs and NCEs in mice bone marrow cells using Micronucleus test, the result shows that incidence of Mn formation in Profenofos treated group were significantly higher than the control .While concurrent treatment with Nyctanthes arbortristis leaves extract with Profenofos shows significantly lower than Profenofos treated group and almost equivalent upto the control level.

Keywords

Profenofos, Nyctanthes arbortristis, Micronucleus, Bone Marrow, Polychromatic erythrocytes, Normochromatic erythrocytes

Conclusion

From above discussion it is conduced that Profenofos is a potent genotoxic agent which induced micronuclei formation and Nyctanthes arbortristis leaves can mitigate its toxicity due to presence of antioxidant richness. Therefore, we must supplement our diet with antioxidants rich herbs. So that toxicity of various pesticides or chemicals can be minimized.

References

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How to cite this article

Rina Kumari and Dharmshila Kumari (2023). Application of Micronucleus Assay for Assessment of Profenofos Genotoxicity and its Mitigation through Nyctanthes arbortristis Leaf Extract in Mice (Mus musculus). Biological Forum – An International Journal, 15(1): 698-700.