Identification of in vitro Regeneration Potential of Rice (Oryza sativa L.) Landraces

Author: Selvamuthu K., Chitra S. and Vanniarajan C.

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Abstract

An efficient callus induction and in vitro regeneration potential of ten rice landraces were analysed along with indica rice variety check ASD 16. The effect of plant growth regulator (2,4-D) on callus induction was studied to maximize the production of embryogenic calli in rice land races. This study found that all the rice land races exhibited high frequency of callus induction on Murashige and Skoog (MS) medium supplemented with 2,4-D 2.5 mg/l. In this concentration, higher callus induction frequency was exhibited by the land race Kallurundikar followed by the landraces Arupatham Kuruvai, Noorungan, Poongar Mattaikar, Kuliyadichan, Chithikaikar, Vellaichithiraikar, Kichalisamba and Nooripathu. The hundred percent regeneration potential was recorded by the land races Chithikaikar, Kallurundikar, Kuliyadichan, Mattaikar and Noorungan when MS media supplemented with BAP 3.0 mg/l + Kinetin 1.5 mg/l + NAA 3.5 mg/l + IAA 1.5 mg/l. Whereas, the landrace Vellaichithiraikar exhibited higher browning rate when compared to other land races. Hence, the optimized conditions for callus formation and regeneration may be used for micropropagation studies for the improvement of rice landraces.

Keywords

Landraces, Rice, Callus, Growth Regulator, In vitro, Shoot regeneration, Rooting

Conclusion

Based on above experimental results, it could be concluded that the yield improvement through biotechnological techniques is feasible by scutellum derived callus and it regeneration in rice land races.

References

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How to cite this article

Selvamuthu K., Chitra S. and Vanniarajan C. (2023). Identification of in vitro Regeneration Potential of Rice (Oryza sativa L.) Landraces. Biological Forum – An International Journal, 15(9): 619-622.