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Effect of Different Culture Media on Growth of Alternaria Isolates

Author: Manisha Solanki, J.J. Kadam and Jeetu Narware

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Abstract

Alternaria blight is a devastating disease among the different cultivated and wild host. Diseases caused by Alternaria are prevalent in all agro climatic zones of Maharashtra. A number of disease cause by Alternaria spp. to cereals, vegetables, fruit, and ornamental crops with huge crop loss. A large variation observed in genus Alternaria with respect of symptom development, morphological characteristics and radial growth. In this experimental study we collected the 58 samples, out of which 26 isolates were taken for the variability study of Alternaria spp. The findings of this study highlight the variation observed in mean radial mycelial growth and dry mycelial weight of Alternaria spp on six different media (PDA, OMA, RAM, AHM, SAM and V8 juice agar). It was recorded that the maximum mean radial mycelial growth of Alternaria isolates was observed on PDA medium followed by OMA medium and minimum growth was recorded on V8 medium. Whereas highest dry mycelial weight was recorded on PDA the and lowest mean dry mycelial weight was recorded on V8.

Keywords

Alternaria spp., radial mycelial growth, dry mycelial weight and six different media.

Conclusion

In this study twenty-six isolates of Alternaria were studied for their cultural variation on different media which are isolated from different host plants. Among the six solid and liquid media evaluated to record colony diameter and dry mycelial weight, the maximum mean radial mycelial growth and dry mycelial weight was observed on PDA medium (Fig. 1a, 1b, 1c, 1d). The least mean mycelial growth and dry mycelial weight was observed on vegetable juice agar (V8) medium (Fig. 2a, 2b, 2c, 2d). The present study will be helpful in the research being carried out by different workers studying pathogen Alternaria to understand its variation in cultural characteristics and its related studies.

References

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How to cite this article

Manisha Solanki, J.J. Kadam and Jeetu Narware (2023). Effect of Different Culture Media on Growth of Alternaria Isolates. Biological Forum – An International Journal, 15(9): 805-812.