Green Synthesis of Nanoparticles from Leaves of Cannabis sativa L. and its effect on Seed Germination, Seedling Mortality and wilt incidence of Tomato caused by Fusarium oxysporum f. sp. lycopersici (Sacc.) Snyder & Hansen

Author: Saurabh Kumar, S.K. Biswas, Kishan Lal, Shivam Kumar, Anju Shukla, Anoop Kumar, Siddharth Singh, Shivam, Ankit Kumar and Rahul Kumar

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Abstract

Now-a-days, crop pest management totally depends on synthetic agro-chemicals and causes harmful residual effect on human and environmental health. There is a rising demand for eco-friendly non-toxic synthetic biological protocol for the synthesis of nanoparticles. The experiment's objective was to create silver nanoparticles from fresh leaves of Cannabis sativa L., characterise them, and test their antifungal properties under in vitro condition. The fungus Fusarium, which is mostly a soil-born pathogen but occasionally also appears on seeds and negatively impacts their viability and germination, is the cause of tomato wilt. Green synthetic nanoparticles greatly enhanced the germination rate and decreased seed mortality in tomato seeds. The highest germination 92.66% found in seed treated with silica nanoparticles at 100 ppm (T12) compared to non-treated plants with the value of 54%. Rest all nanoparticles treated seed was representing superior than non- treated seeds. The minimum seedling mortality as 5.25% was found in T12 (silica NPs 100 ppm) than all treated and non-treated seeds. The disease incidence was decreased over control as 86.66%, 86.00% and 83.32% at 30, 60 and 90 days of pathogen inoculation, respectively was observed in treatment T12. It was established that all plants treated with nanoparticles lower disease incidence than the control. In general, nanoparticles work to combat plant infections by altering root exudates and starting the release of stress hormones. Because of their varied and distinctive mechanisms of action, nanoparticles can suppress pathogens that cause wilt in sustainable manner.

Keywords

Nanoparticles, germination, seedling mortality, stress, wilt and pathogen

Conclusion

On account of eco-friendly, biodegradable, non-chemicals more efficient in nature and the increasing trends of green synthesis nanoparticles in near future may be prove best substitute instead of chemical pesticides against plant disease management. The seed treated with green synthesized nanoparticles was positively raising germination (%) and reduces the mortality percentage of tomato seedling. All plant treated with nanoparticles significantly increased the root, shoot length and total biomass of the plant. The Treatment T12 (silica 100 ppm) treated plant reduces 86.66%, 86.00% and 83.32% disease incidence over the control at 30, 60 and 90 days of pathogen inoculation, respectively. Thus, it is evident from the current evaluation that the use of green synthesis nanoparticles can be employed to manage tomato wilt in sustainable manner.

References

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How to cite this article

Saurabh Kumar, S.K. Biswas, Kishan Lal, Shivam Kumar, Anju Shukla, Anoop Kumar, Siddharth Singh, Shivam, Ankit Kumar and Rahul Kumar (2023). Green Synthesis of Nanoparticles from Leaves of Cannabis sativa L. and its effect on Seed Germination, Seedling Mortality and wilt incidence of Tomato caused by Fusarium oxysporum f. sp. lycopersici (Sacc.) Snyder & Hansen. Biological Forum – An International Journal, 15(9): 850-855.