Bio-Efficacy of different Botanical extracts against Fusarium oxysporum f. sp. lentis, causing lentil wilt under in-vitro condition

Author: Karan Singh, Meenu Kumari Meena, C.B. Meena, Chirag Gautam and Hemant Gurjar

Download PDF

Abstract

Present investigation was undertaken for bio-efficacy of aqueous botanical extracts of eight different non-host plants viz., Azadirachta indica (NSKE), Azadirachta indica (Leaves), Osimum sanctum (Leaves), Datura stramonium (Leaves), Calotropis procera (Leaves), Lantana camara (Leaves), Nerium oleander (Leaves) and Parthenium histrophorus (Leaves) at 5, 10 and 20% concentration were evaluated against Fusarium oxysporum f. sp. lentis, causing lentil wilt through poison food technique on potato dextrose agar medium. The result revealed that Among all eight botanical extracts, extract of Osimum sanctum (Leaves) was found most effective in inhibiting mycelial growth (21.48, 30.93 and 45.19 %) of F. orysporum f. sp. lentis at 5, 10 and 20 percent, respectively followed by Azadirachta indica (NSKE) extract (19.26, 28.52 and 41.85%) over control at all the concentrations tested after 168 hrs. of inoculation. At 5 per cent concentration Calotropis procera (Leaves) and Azadirachta indica (Leaves) were found at par with 10.74% and 12.96% mycelial growth inhibition, respectively, Extract of Parthenium histrophorus (Leaves) was found least effective in inhibiting mycelial growth of Fusariumo xysporum f. sp. lentis over control 4.07, 10.93 and 17.59 at 5, 10 and 20 percent concentration, respectively.

Keywords

Botanical extracts, Neem Seeds Kernel Extract (NSKE), Fusarium orysporum f. sp. lentis, Poison food technique

Conclusion

Botanical’s control is very important aspects to minimize cost of cultivation and to avoid environment pollution and health hazards. The result revealed that Among all eight botanical extracts, extract of Osimum sanctum (Leaves) was found most effective in inhibiting mycelial growth (21.48, 30.93 and 45.19 %) of F. orysporum f. sp. lentis at 5, 10 and 20 percent, respectively followed by Azadirachta indica (NSKE) extract (19.26, 28.52 and 41.85%) over control at all the concentrations tested after 168 hrs. The results however need field evaluation before these recommended to farmers.

References

-

How to cite this article

Karan Singh, Meenu Kumari Meena, C.B. Meena, Chirag Gautam and Hemant Gurjar (2023). Bio-Efficacy of different Botanical extracts against Fusarium oxysporum f. sp. lentis, causing lentil wilt under in-vitro condition.. Biological Forum – An International Journal, 15(9): 882-886.