Development and Biological Evaluation Transdermal Hydrogel for the Controlled Delivery of Curcumin against Atopic Dermatitis Management: In-Vitro and In-Vivo Studies

Author: Umakant Sharma, Dindayal Patidar and Neeraj Sharma

Download PDF

Abstract

Targeting atopic dermatitis has always been a challenging task due to complex genetic phenomena and conflicting biochemical microenvironments. Drug delivery via the skin against atopic dermatitis is always been the priority based mechanism due to patient compliance and low local toxicity. By utilising lauric acid as the plasticizer, a transdermal chitosan sodium alginate biodegradable polymer mix hydrogel (CCHL) has been developed to improve the biotherapeutic properties of curcumin. Utilizing lauric acid at an optimised concentration for the physicochemical and thermostable formulation, the CCHL was prepared utilising the cold technique. When developing the biologically stable hydrogel, the optimised hydrogel was physiologically assessed using in vitro cell uptake, cell viability, and apoptosis assays for a complete toxicological and biologically safe margin description. The MTT assay and Apoptosis study of the produced hydrogel revealed qualitative results that were physicochemical and biologically compatible, demonstrating the considerable curcumin occupancy on human dermal fibroblast cell. By virtue of the anti-AD action, bio-pharmacokinetic parameters, histopathology and skin erythema impact, the in vivo DNCB based animal experiments revealed that the produced hydrogel was physiologically safe for on transdermal distribution. Overall, the current work successfully established regulated curcumin transdermal administration as a unique route to physiologically safe nanotherapy in a clinical setting. Additionally transdermal anti AD targeting comprehending natural model drug and eco-friendly delivery carrier made possibilities more contingent for the futuristic management of AD and its treatment.

Keywords

Hydrogel, Transdermal, in-vitro cell line, toxicology, In-vivo

Conclusion

The results demonstrate the qualitative penetrating potential of biodegradable cationic chitosan loaded curcumin hydrogel offering pH triggered and charge rebound innate mechanism enabling microenvironment dependent anti AD therapeutic effect and improving curcumin release with decreased local cellular toxicity. As shown by in vitro cell line studies and hematological studies therapeutic transdermal targeting evaluation, ionic interaction and EPR dependent absorption of CCHL enable onsite targeting and subsequent toxicity on HDF cell line, lowering atopic dermatitis infection burden on skin area. Discovering a unique transdermal biodegradable therapy with harmless constituent’s usage the advanced in-vivo evaluation showed increased diffusion of CCHL and optimal retention at the targeted dermatitis location with negligible cellular toxicity at low dose of curcumin. The fabricated hydrogel demonstrated the most extreme Anti-AD specificity with lowered dose and duration, improving therapeutic efficacy for atopic dermatitis patients with increased skin rehabilitations rate, and paving the way for simple and effective clinical transdermal nanotherapy against atopic dermatitis.

References

-

How to cite this article

Umakant Sharma, Dindayal Patidar and Neeraj Sharma (2023). Development and Biological Evaluation Transdermal Hydrogel for the Controlled Delivery of Curcumin against Atopic Dermatitis Management: In-Vitro and In-Vivo Studies. Biological Forum – An International Journal, 15(5a): 592-602.