GC-MS Analysis of Volatile Phytochemical compounds from the Whole plant Methanolic extract of Entodon rubicundus (Mitt.) A. Jaeger. and Thyridium fasciculatum (Hook. & Grev.) Mitten

Author: Shiwani Latwal, Meenal Sharma and Anju Rao

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Abstract

Medicinal plants are the most formidable chemical laboratory of bioactive compounds attributed with healing properties against various ailments. The phytochemistry of the plants has been exploited to satisfy the growing demand for therapeutic needs. When it comes to potential medical use, bryophytes are among the most neglected land plants. Using the gas chromatography-mass spectrometry (GC-MS) technology, the study sought to assess potential phytochemical components and their medicinal importance in the aqueous methanol fraction of plants. Entodon rubicundus (Mitt.) A. Jaeger and Thridium fasciculatum (Hook. & Grev.) Mitten are two mosses from the Western Himalayan region belonging to the family Entodontaceae and Calymperaceae respectively. The samples were collected during their favourable season, washed, air- dried and then macerated to produce the methanolic extract. GC- MS analysis was carried out followed by the identification of volatile compounds using mass spectra library search (NIST 2.0). Five volatile constituents were detected and identified in the plant extract of Entodon rubicundus out of which Trans-13-Octadecenoic acid, methyl ester showed the highest peak area (46.58%) and the lowest peak area was shown by Methyl stearate (4.10%). In Thridium fasciculatum seven compounds were identified out of which Cyclopropaneoctanoic acid showed the highest peak area (44.95%) and 6,9 Octadecadienoic acid, methyl ester showed lowest the peak area (1.68%). To the best of our knowledge, no literature has previously been published on GC–MS and phytochemical investigations of these moss species, making it the first description of the phytochemical elements that demonstrate their pharmacological importance. The GC- MS profiling of both the mosses indicated therapeutically promising bioactive compounds with a potent anti-bacterial, anti-microbial, anti-tumor, anti-inflammatory and anti-oxidant activity which can be used in pharmaceutical industries for the development of novel drugs.

Keywords

Entodon rubicundus, Thyridium fasciculatum, Mosses, GC-MS, Phytochemicals

Conclusion

The pharmacological use of plants as a source for the development of innovative drugs has gained widespread recognition in the last few years. To gain a thorough understanding of the phytochemical profile of the mosses found in the Western Himalayan region, a GC-MS analysis of the mosses in a methanolic solvent was conducted. The resulting compounds displayed a wide range of biological characteristics, including anti-inflammatory, anti-bacterial, anti-acne, anti-tumor, anti-cancerous, anti-oxidant, and anti-arthritic capabilities. Based on the conducted research, it is possible to conclude that both E. rubicundus and T. fasciculatum could be recommended as a plant of pharmaceutical importance.

References

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How to cite this article

Shiwani Latwal, Meenal Sharma and Anju Rao (2023). GC- MS Analysis of Volatile Phytochemical compounds from the Whole plant Methanolic extract of Entodon rubicundus (Mitt.) A. Jaeger. and Thyridium fasciculatum (Hook. & Grev.) Mitten. Biological Forum – An International Journal, 15(5a): 647-653.