Effect of in situ Action of Serotonin in Transmembrane Ion Transport in Mice Exposed to Restraint Stress

Author: Manish, K., Sajeeb Khan, A., Praveen Kumar P.P.,Nandini N.J. and Ayana Gayathri R.V.

Download PDF

Abstract

Serotonin (5-HT) is known to be a key modulator of stress effects. However, its role in ion transport during stress response is not yet understood. The dose-dependent effect of in situ serotonin was examined in Swiss albino mice. 10-9 M 5-HT perfusion dose seems to be effective in producing a pronounced effect on most of the tissues. Perfusion of serotonin at 10-9 M for 20 minutes produced a significant decrease in Na+, K+-ATPase activity in the kidney, liver, stomach and intestinal tissues. A dose-responsive decrease in cytosolic and mitochondrial H+ ATPase activity was found in these tissues after serotonin perfusion. Likewise, the cytosolic and mitochondrial Ca2+ ATPase activities decreased in the kidney, liver, stomach and intestine. The mitochondrial Mg2+ ATPase activity decreased in the tested tissues in a dose-responsive manner. Subjecting mice to restraint stress for seven days increased the Na+, K+-ATPase, H+ ATPase, Ca2+ ATPase and Mg2+ ATPase activities to significant levels in kidney, liver, stomach and intestinal tissues. On the contrary, in-situ perfusion of serotonin to stressed mice at 10-9 M caused decrease in the stress-induced hyperactivity of these transmembrane ion transporters. The above results show a role of serotonin in ion transporter activity and suggest the mitigation role of serotonin in ion transport during stress response in mice.

Keywords

ATPase, Serotonin, Mice, Stress

Conclusion

-

References

-

How to cite this article

Manish, K., Sajeeb Khan, A., Praveen Kumar P.P.,Nandini N.J. and Ayana Gayathri R.V. (2023). Effect of in situ Action of Serotonin in Transmembrane Ion Transport in Mice Exposed to Restraint Stress. Biological Forum – An International Journal, 15(3): 839-850.