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Sperm Morphological Abnormalities in Fresh Semen of Marwari Stallion

Author: Vishal Yadav, Thirumala Rao Talluri, Sandeep Dholpuria, Sajjan Kumar, Abhishek Joshi, Shivali Khandelwal and Ashok Kumar

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Abstract

When it comes to predicting future fertility, sperm morphology might even be more significant than motility or count. Sperm with an irregular shape cannot fertilise an egg. Spermatozoa may have a combination of abnormalities. The present study was carried out on six healthy Marwari horses with a semen collection frequency of twice a week. Total 36 (6 per animal) ejaculates were collected using artificial vagina. After collection, semen samples were evaluated grossly, followed by microscopic evaluation. The assessment of sperm morphologies in fresh equine semen was conducted using the eosin and nigrosin staining technique. Morphological abnormalities in the head, mid-piece and tail were 1.42±0.12%, 1.44±0.14% and 1.56±0.10%, respectively and total sperm morphological abnormalities were 4.42±0.23%. Morphological sperm abnormalities (head, mid-piece and tail) along with total sperm morphological abnormalities exhibited a non-significant (P>0.05) difference among Marwari stallions.

Keywords

Equine semen, fertility, Marwari stallion, sperm morphology, sperm abnormalities

Conclusion

The present study concludes the morphological abnormalities of sperm present in the fresh semen of Marwari stallions during the breeding season, including abnormalities in the sperm head, mid-piece and tail, along with total sperm morphological abnormalities. All the morphological abnormalities exhibited a non-significant difference (P>0.05) among Marwari stallions. The effects of environmental factors like weather and breeding season and the effects of age and frequency of semen collection on the morphological abnormalities of sperm should be studied to know the factors influencing sperm morphological abnormalities of Marwari stallion.

References

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How to cite this article

Vishal Yadav, Thirumala Rao Talluri, Sandeep Dholpuria, Sajjan Kumar, Abhishek Joshi, Shivali Khandelwal and Ashok Kumar (2024). Sperm Morphological Abnormalities in Fresh Semen of Marwari Stallion. Biological Forum – An International Journal, 16(1): 37-40.