Identification of Suitable Solvent for Extraction of Principle Insecticidal compounds from Selected Medicinal and Aromatic Plants and Evaluation of their Efficacy against Plutella xylostella (L.)

Author: Kamala Devappa Gaddennavar, Gangadhar Narabenchi, B.N. Maruthi Prasad and Jayashree Ugalat

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Abstract

A laboratory experiment was carried out to identify a suitable solvent for extraction of principle insecticidal compounds from selected medicinal and aromatic plants. Further, these insecticidal compounds were evaluated for their efficacy against diamondback moth, Plutella xylostella (L.). Four different solvents such as methanol, chloroform, ethyl acetate and water were used to extract insecticidal compounds from the four plants namely, neem, pongemia, datura and bael. Based on the LC50 and LT50 values registered with four solvents and four plants, the order of suitability of solvent in extracting the insecticidal principle compounds from the plants is as follows; chloroform > aqueous > methanol > ethyl acetate. When the different plants are considered for their efficacy irrespective of the solvents used for extraction of insecticidal principle compounds from them, the order of effectiveness is as follows; neem > datura>bael>pongamia.

Keywords

DBM, solvents, LC50, LT50, medicinal and aromatic plants extract, bioassay, Soxhlet’s extractor and magnetic stirrer

Conclusion

Among the different solvents such as methanol, ethyl acetate, chloroform and water evaluated for their suitability in extracting maximum amount insecticidal compounds from test plants, the chloroform extract of neem seed kernels showed highest toxicity by registering lowest LC50 and LT50 values against early third instar larvae. Next best plants in the order of toxicity were seed extract of pongamia, leaf extract of datura and bael. It can be concluded from the present investigation that, the chloroform extract found more suitable solvent in extracting maximum amount of insecticidal compounds from the medicinal and aromatic plants as that of other solvents tested.

References

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How to cite this article

Kamala Devappa Gaddennavar, Gangadhar Narabenchi, B.N. Maruthi Prasad and Jayashree Ugalat (2024). Identification of Suitable Solvent for Extraction of Principle Insecticidal compounds from Selected Medicinal and Aromatic Plants and Evaluation of their Efficacy Against Plutella xylostella (L.). Biological Forum – An International Journal, 16(1): 239-242.