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Effect of 2,3,7,8-Tetrachlorodibenzo-p-dioxin Toxin on Cell viability in Cultured Caprine Granulosa Cells

Author: Femi Francis, Raji Kanakkaparambil, Ramziya P.K., Pratheesh Mankuzhy, Babitha Vazhur, Roshin Anie Jose and Sanis Juliet

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Abstract

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), a highly potent dioxin toxin, is being discharged into the environment through various human activities. Notably, it possesses the ability to bioaccumulate in adipose tissues. TCDD is recognised for its detrimental impact on reproductive functions. The present study reports that exposure to different doses of TCDD in vitro, even at higher concentrations, did not affect the viability of granulosa cells. The study tested the hypothesis that exposure to TCDD ranging from 5 nM to 100 nM could affect granulosa cell viability in a dose-dependent manner. For testing the hypothesis, granulosa cells isolated from the caprine ovaries were used for the study. The isolated granulosa cells were divided into a control group and three treatment groups and were exposed to different doses of TCDD (5 nM, 10 nM, and 100 nM) for 24 hours. The observed results indicated that cell viability was maintained in the granulosa cells exposed to all the concentrations of TCDD examined. A non-significant dose-dependent decrease was also observed in the study. The present study suggested that the TCDD doses studied might not be cytotoxic enough to decrease the cellular viability of the granulosa cells for 24 hours.

Keywords

TCDD, goat, granulosa cells, cell viability, dose-dependent

Conclusion

Overall, the results of the present study indicated that the viability of granulosa cells decreased in a dose-dependent manner in response to different doses of TCDD, although the studied TCDD doses were not toxic enough to significantly reduce cell viability. The potential harm to cell viability might have been averted due to either a brief exposure to TCDD or variations in species response to it.

References

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How to cite this article

Femi Francis, Raji Kanakkaparambil, Ramziya P.K., Pratheesh Mankuzhy, Babitha Vazhur, Roshin Anie Jose and Sanis Juliet (2024). Effect of 2,3,7,8-Tetrachlorodibenzo-p-dioxin Toxin on Cell viability in Cultured Caprine Granulosa Cells. Biological Forum – An International Journal, 16(2): 15-19.