GCMS Analysis of Phytoconstituents in Leaf Extract of Drypetes roxburghii and its Neuroprotective Potential

Author: Mohd Afzal, Mohammad Asif and Abdul Hafeez

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Abstract

The study deals with the determination of neuroprotective effect of crude extract Drypetes roxburghii leaves. In this study, the crude extract of leaves of D. roxburghii was obtained and the phytochemical composition was analyzed using GC-MS. Afterwards, the acute oral toxicity of the extract was performed using OECD 420, based on which two doses, 100 and 200 mg/kg BW, were selected for further pharmacological evaluation. The Neuroprotective activity of the extract was evaluated in regard to its anti-epileptic, anti-anxiety, anti-depressant, muscle relaxant, and anti-oxidant activity. The mentioned activities were performed using various experimental animal models. The data was analyzed using graph pad prism, version 9. P<0.05 was selected as criteria of statistical significance. The results of the study revealed that the crude extract was found to be rich in many medicinally important constituents such as 5-ethylhydantoin, cis-vaccenic acid, and phenol, 2, 4-bis (1, 1- dimethylethyl). The extract possessed significant anti-epileptic, anti-anxiety, muscle relaxant, and anti-oxidant activity but not anti-depressant activity. The effect of test dose 200 mg/kg, BW was comparable to that of standard drug. Based on the result it can be concluded that the crude extract of D. roxburghii leaves contains constituents responsible for its neuroprotective potential.

Keywords

Drypetes roxburghii, GCMS analysis, anti-epileptic, anti-anxiety, anti-depressant, muscle relaxant, and anti-oxidant activity

Conclusion

The hydroalcoholic crude extract of Drypetes roxburghii leaves revealed the presence of 22 compounds. When the extract was evaluated for its Neuroprotective activity, it showed significant anti-epileptic, anti-anxiety, muscle relaxant, and anti-oxidant activity. Among the identified compounds 5-Ethyl hydantoin, pentadecanoic acid, Cis-vaccenic acid, 9-octadecenoic acid, and 2,4-bis (1,1, dimethyl ethyl) are thought to be responsible for the above mentioned activities.

References

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How to cite this article

Mohd Afzal, Mohammad Asif and Abdul Hafeez (2023). GCMS Analysis of Phytoconstituents in Leaf Extract of Drypetes roxburghii and its Neuroprotective Potential. Biological Forum – An International Journal, 15(6): 819-831.