Indexing

Diversity Guided Antibacterial Bioactive Metabolites from Endophytic Fungi of Withania somnifera (L.) Dunal

Author: Shubha Jagannath, Hanna Tayeng, Sushmita Iswary, Ashalatha K.S., Narasimhamurthy Konappa, Srinivas Chowdappa and Raveesha Haranahalli Ramaiah

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Abstract

Withania somnifera (L.) Dunal, is commonly acknowledged as ashwagandha or as Indian ginseng. It is used in Indian system of traditional medicine and it exhibits broad spectrum therapeutic properties. The sum of 165 endophytic fungi belonging to twenty-two different species was isolated from 250 tissue fragments analyzed. The Shannon and Simpson diversity indices were greater in stem trailed by root, leaf, flower and seed. The phytochemical analysis showed that 91% of endophytic fungal isolates produced terpenoids, 60% flavonoids, 42% steroids, 40% quinones and 54% tannins. Further, fungal isolates were analyzed by agar plug method for antibacterial activity; Talaromyces radicus (WSR2) and Aspergillus niger (WSR1) isolated from root of W. somnifera exhibited broad range activity against all the Gram-positive and Gram-negative pathogenic bacteria tested. These two endophytic fungi were subjected to molecular identification and sequences submitted to Gen Bank and accession numbers obtained Talaromyces radicus - MN099424.1, Aspergillus niger - MN099425.1. The GC-MS analysis of Talaromyces radicus (WSR2) ethyl acetate crude extract demonstrated the existence of eighteen compounds.

Keywords

Endophytic fungi, Diversity, Bioactive metabolites, Withania somnifera

Conclusion

The present results revealed that the endophytic fungi associated with the medicinal plants are tremendous reservoirs of many bioactive metabolites which can be used for the betterment of mankind and nature.

References

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How to cite this article

Shubha Jagannath, Hanna Tayeng, Sushmita Iswary, Ashalatha K.S., Narasimhamurthy Konappa, Srinivas Chowdappa and Raveesha Haranahalli Ramaiah (2023). Diversity Guided Antibacterial Bioactive Metabolites from Endophytic Fungi of Withania somnifera (L.) Dunal. Biological Forum – An International Journal, 15(6): 846-863.