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Antiproliferative Effect of Fibrinolytic Enzyme and Apoptotic Gene Expression Studies on Human Carcinoma Cell Lines Produced from the Microorganism of Poultry Farm

Author: Subha T., Sreeya G. Nair and Beena Somanath

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Abstract

Cancer is one of the serious disease, causes millions of human deaths worldwide and hence it is one of the most investigated research disciplines. The present study focuses on the antiproliferative effect and apoptotic gene expression of the fibrinolytic enzyme against cancer cell line. A newly isolated strain with fibrinolytic activity was screened from dumped soil enriched with poultry waste and the fibrinolytic enzyme produced in the optimised medium was purified. The final specific activity of the purified enzyme was found to be 1033.23 units/mg, which indicated that the enzyme was purified 21.08 fold with approximately 25% yield. Antiproliferative effect of the fibrinolytic enzyme on HeLa cell line revealed that the cell viability decreased with increased concentration of the enzyme. Apoptotic studies of fibrinolytivc enzyme showed that the gene expression of Bax was increased and Bcl2 activity was decreased on cancer cell. The produced fibrinolytic enzyme showed its maximum anticancerous activity by decreasing the viability of cancer cells. In future this research area may result in the creation of using this novel fibrinolytic enzyme for the treatment of cancer.

Keywords

Poultry waste, Optimised medium, anti-proliferative effect, apoptotic studies, gene expression

Conclusion

Our data exhibited that the crude fibrinolytic enzyme purified from poultry farm has potential antiproliferative effect on cancer cell and also revealed significant expression of apoptotic genes on cancer cell.

References

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How to cite this article

Subha T., Sreeya G. Nair and Beena Somanath (2023). Antiproliferative Effect of Fibrinolytic Enzyme and Apoptotic Gene Expression Studies on Human Carcinoma Cell Lines Produced from the Microorganism of Poultry Farm. Biological Forum – An International Journal, 15(3): 867-871.