Impact of Plant Bioregulators on In Vitro Callogenesis of Gerbera Hybrid cv. Alcatraz

Author: Subhadarsini Pradhan and Sashikala Beura

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Abstract

In vitro cloning of gerbera for true-to-type and disease free plants has been preferred over conventional vegetative propagation for large scale production of plantlets. An experiment was conducted at Biotechnology-cum –tissue culture centre of OUAT, Bhubaneswar with the objective to establish protocol for callusing in gerbera hybrid cv. Alcatraz through in vitro cloning using capitulum as an explant. The MS medium was used as the base and was added with varying concentrations of BAP, IAA, and NAA in various combinations to create callus emergence media. Proper sterilisation process was followed for maintaining aseptic environment at in vitro cloning. The experiment revealed that T6 (MS + BAP 2.0mg/l + IAA 0.5mg/l) produced callus earlier (18.20 days) with longer callus development days (27.10 days) and larger callus spread (4.03cm2) of compact and greenish white callus after 45 days of inoculation. However, T2 (MS + BAP 1.0mg/l) recorded the lowest values for all parameters. After 1st sub-culturing T7 (MS + BAP 3.0mg/l + IAA 0.5mg/l) was found best for all the parameters which was also showed at par effect with T6 (MS + BAP 2.0mg/l + IAA 0.5mg/l)

Keywords

Gerbera, Alcatraz, Callus formation, In vitro culture

Conclusion

The experiment on callogenesis of Gerera cv. Alcatraz concluded that quarter of capitum can be used to produce callus in vitro which could produce healthy plantlets on large scale in less time. MS media enhanced with BAP 2.0mg/l and IAA 0.5mg/l resulted best for callus formation and development

References

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How to cite this article

Subhadarsini Pradhan and Sashikala Beura (2023). Impact of Plant Bioregulators on In Vitro Callogenesis of Gerbera Hybrid cv. Alcatraz. Biological Forum – An International Journal, 15(1): 773-776.