Effect of Pesticide’s Residue on the Physiological Changes in the Silkworm (Bombyx mori L.) Haemolymph

Author: Sandhya N., N. Amarnatha, K.C. Narayanaswamy, Manjunath Gowda and N. Nagesha

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Abstract

The organic constituents of haemolymph (enzymes) play an important role in biochemical processes underlying growth and development of insects. The study on haemolymph of silkworm (Bombyx mori L.) during the fifth instar revealed that pesticide exposure affects haemolymph enzyme activities, crucial for growth and development. Amylase activity in control groups (water spray, absolute control) increased until day 5, then declined, while pesticide treatments caused an early peak and subsequent sharp decline. Acetylcholinesterase (AChE) activity rose steadily in the carbofuron 3G treatment but was dampened with novaluron 10EC exposure. Peroxidase (POX) activity spiked initially under pesticides but dropped significantly by day 6. Novaluron 10EC and chlorfenapyr 10SC were notably toxic, affecting enzyme activity even after a waiting period

Keywords

Silkworm, peroxidase, amylase, acetylcholinesterase, haemolymph, pesticides

Conclusion

The study concludes that pesticide exposure significantly impacts enzyme activities in silkworm haemolymph. Amylase activity in control groups peaked on day 5, while pesticide-treated groups showed an early rise followed by a decline. Acetylcholinesterase (AChE) activity steadily increased with carbofuron 3G but was dampened by novaluron 10EC. Peroxidase (POX) activity initially spiked under pesticides, then decreased sharply. Control treatments showed minimal enzyme fluctuations. Overall, pesticides like novaluron 10EC and chlorfenapyr 10SC proved highly toxic, disrupting enzyme activities

References

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How to cite this article

Sandhya N., N. Amarnatha, K.C. Narayanaswamy, Manjunath Gowda and N. Nagesha (2024). Effect of Pesticide’s Residue on the Physiological Changes in the Silkworm (Bombyx mori L.) Haemolymph. Biological Forum – An International Journal, 16(8): 225-231.