Evaluation of Phytochemical Analysis and Antibacterial Activity of Chara fibrosa and Cladophora crispata

Author: Sunil D. Rathwa and Vasant A. Patel

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Abstract

This study evaluates the antibacterial activities of various solvent extracts from the freshwater algae Chara fibrosa and Cladophora crispata. The agar well diffusion method was utilized to evaluate the antibacterial activity against three types of bacteria, namely E. coli, S. marcescens and S. aureus. The inhibition zones measured for all crude extracts demonstrated a broad spectrum of antimicrobial activity against the tested pathogens. The fresh water algal biomass's FTIR spectrum investigation revealed the existence of organic chemical groups like C-Br, C-N, C-O and C=C. These findings suggest that the biomass of Chara fibrosa and Cladophora crispata constitutes a sustainable green resource. The observed antibacterial activity implies that the active constituents within the freshwater algae extracts can be harnessed for the development of innovative pharmaceuticals, potentially benefiting both medical and agricultural applications

Keywords

freshwater algae, FTIR, antibacterial activity, agar well diffusion method

Conclusion

This research provides a preliminary investigation into the antibacterial potential of the freshwater microalgae Chara fibrosa and Cladophora crispata, assessing their efficacy against prominent bacterial pathogens including Escherichia coli, Serrati amarcescens, and Staphylococcus aureus. The antibacterial activity of the algal extracts was assessed using the agar well diffusion method in this investigation. Which demonstrated notable effectiveness against E. coli and S. marcescens, but no activity against S. aureus. The results highlight the significant antibacterial properties of these microalgae, attributable to the presence of bioactive compounds such as phenolics, amines, aromatic cyclic compounds, and halo-compounds. These compounds were identified through FTIR analysis, revealing diverse functional groups that potentially contribute to the observed antimicrobial activity (Suciyati et al., 2021). Specifically, Cladophora crispata exhibited superior antibacterial activity compared to Chara fibrosa, particularly against S. marcescens, indicating a higher efficacy of the former in inhibiting bacterial growth. This study underscores the potential of utilizing natural phycocompounds from these algal species in the drug discovery process. The promising antibacterial activity observed suggests that Chara fibrosa and Cladophora crispata could serve as valuable sources of novel antimicrobial agents (Asmaa et al., 2021).

References

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How to cite this article

Sunil D. Rathwa and Vasant A. Patel (2023). Evaluation of Phytochemical Analysis and Antibacterial Activity of Chara fibrosa and Cladophora crispata. Biological Forum – An International Journal, 15(3): 916-922