Screening and Identification of Cellulolytic Streptomyces viridochromogenes Strain LPA75 in Cotton Crop Compost from Beed, Maharashtra

Author: L.N. Chavan, M.D. Shaker, A.V. Deshpande and P.P. Dixit

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Abstract

Cellulose is the most abundant carbohydrate in nature and the main structural component of plant cell walls. Cellulases, enzymes that break down cellulose, hold significant industrial potential. This study successfully isolated and identified a cellulolytic strain, S. viridochromogenes LPA75, from cotton agricultural waste compost (CAWC) in Beed, Maharashtra. The strain exhibited strong cellulase activity, making it a promising candidate for degrading cellulose-rich agricultural waste. Morphological, biochemical, and molecular characterization, including 16S rRNA gene sequencing, confirmed its identity. S. viridochromogenes LPA75 shows potential in sustainable organic agriculture, enhancing crop productivity and soil health in cotton-growing regions. The investigation not only highlights its enzymatic abilities but also the value of bacterial diversity in agro-waste ecosystems. After optimization, S. viridochromogenes LPA75 could be scaled up for eco-friendly waste recycling and nutrient management, reducing reliance on chemicals. This study presents a breakthrough in bio-organic agricultural strategies, offering a green approach to improve soil fertility and productivity, paving the way for future research into its industrial-scale applications

Keywords

Cellulose, Actinobacteria, 16S rRNA gene sequencing, Polyphasic approach, S. viridochromogenes LPA75

Conclusion

The successful isolation and characterization of S. viridochromogenes strain LPA75 from cotton crop compost in Malpuri Village of Beed, represent/potential of agricultural waste as a promising reservoir of industrially important enzymes, particularly CAWC which is rich in complex cellulose. This strain’s cellulolytic capacities hold considerable promise for sustainable agriculture, particularly in accelerating composting efficiency and facilitating the decomposition of agricultural biomass. The identification of strain LPA75 adds to the growing body of evidence underlined the role of Streptomyces species in agro-biotechnological applications. Globally, increasingly prioritizing eco-friendly and sustainable practices, the findings of this study could serve as a cornerstone for innovative approaches to waste management and agricultural productivity. Future studies could explore optimizing the enzymatic activity of this strain LPA75 and its applications in different agroeconomic processes. Further work needs to be done for purification of cellulase enzyme by column chromatography, determining the enzyme activity and protein content in purified culture extracts followed by peptide profiling by using Mass spectrometry. Thus, the discovery of S. viridochromogenes strain LPA75 represents a significant step toward achieving a more sustainable and productive agricultural ecosystem

References

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How to cite this article

L.N. Chavan, M.D. Shaker, A.V. Deshpande and P.P. Dixit (2023). Screening and Identification of Cellulolytic Streptomyces viridochromogenes Strain LPA75 in Cotton Crop Compost from Beed, Maharashtra. Biological Forum – An International Journal, 15(3): 923-929