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Identification of Egg Development Genes in Meloidogyne incognita as Potential Targets for Nematode Control

Author: Anamika, Manish Kumar, Neeraj and Anil Sirohi

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Abstract

Silencing genes associated with egg development and fertility in Meloidogyne incognita using RNA interference (RNAi) offers a potential avenue for effectively managing this destructive crop pest. To identify potential targets, we selected the gene vit-2 based on its functional role in the model nematode Caenorhabditis elegans. The vit-2 gene, part of the vitellogenin gene family, is essential for yolk production, which supports post-embryonic larval development and fertility. In silico analysis of Meloidogyne incognita using BLASTn and tBLASTx search tools led to the identification of the key target gene, Minc3s00286g09435. Primers for the identified gene were designed using the PrimerQuest tool from IDT/the Primer3 Plus software. The presence of the gene in Meloidogyne incognita was confirmed through polymerase chain reaction (PCR) followed by gel electrophoresis. A partial sequence of 716 bp was obtained for the vit-2 gene. With the confirmed presence of the gene, it can be targeted through RNA interference (RNAi) to investigate its functional role in Meloidogyne incognita. Confirming the gene's function could enable RNAi-based control of Meloidogyne incognita, enhancing sustainable pest management and crop resilience

Keywords

Meloidogyne incognita, RNA interference (RNAi), vit-2 gene, egg development, fertility, PCR, gel electrophoresis, sustainable pest management

Conclusion

In conclusion, this study highlights the distinct role of the vitellogenin-2 (vit-2) gene in nematode development beyond its traditional function in yolk production. The identification of an orthologous gene in Meloidogyne incognita (Minc3s00286g09435) suggests a conserved role for vit-2, potentially linked to the daf-16 pathway. These findings point to vit-2 as a promising target for novel nematode control strategies

References

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How to cite this article

Anamika, Manish Kumar, Neeraj and Anil Sirohi (2023). Identification of Egg Development Genes in Meloidogyne incognita as Potential Targets for Nematode Control. Biological Forum – An International Journal, 15(6): 947-949