The Genomic Landscape of Plant Growth-promoting Rhizobacterium Paenibacillus macerans Strains

Author: Verma K., Srivashtav V., Singh A., Prasad R. and Kumar S.

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Abstract

Paenibacillus macerans is a plant growth-promoting rhizobacterium (PGPR) that promotes plant growth, improves soil fertility, and suppresses plant diseases, while also possessing beneficial traits for DNA metabolism, virulence, disease and defence. Despite its potential, Paenibacillus macerans remains understudied, with limited comprehensive genomic information available. This study utilizes draft genome sequence data of Paenibacillus macerans strains CMB402, CMB401, and CMB393 to investigate their genomic characteristics and comparative analysis with reference sequence 3CT49. The results show that CMB402 exhibits a higher GC content, a higher N50 value, and a lower number of contigs compared to the other two strains. Notably, CMB402, CMB401 harbors three and CMB393 with two antibiotic-resistance genes with similar values and 7285 annotated genes, including those involved in DNA metabolism, virulence, disease and defence. Comparative analysis reveals that CMB402 has undergone fewer genome rearrangements than CMB401 and CMB393. This study provides valuable insights into the metabolic capabilities of Paenibacillus macerans, including antibiotic resistance, and virulence, which can be useful for biologists studying plant metabolism and stress responses.

Keywords

Paenibacillus macerans, Comparative genome analysis, Antibiotic resistance gene, Prophage, Virulence

Conclusion

In summary, the present study has elucidated the genomic characteristics of P. macerans strains CMB402, CMB401, and CMB393, providing valuable insights into their genetic makeup and potential applications. The findings of this study suggest that P. macerans strain CMB402 is a promising candidate for agricultural and bioremediation applications, owing to its possession of genes related to phosphate solubilization, nitrogen metabolism, and antibiotic resistance. Furthermore, the high-quality draft assembly and intact prophages of CMB402 indicate its potential for genetic stability and adaptability. While P. macerans CMB393 exhibits a robust defensive system, as evidenced by its 17 CRISPR array-encoded genes, CMB402's overall genomic characteristics make it an attractive candidate for further research and development. Collectively, these findings contribute to our understanding of the genetic diversity of P. macerans and highlight the potential of this species for various biotechnological applications.

References

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How to cite this article

Verma K., Srivashtav V., Singh A., Prasad R. and Kumar S. (2024). The Genomic Landscape of Plant Growth-promoting Rhizobacterium Paenibacillus macerans Strains. Biological Forum – An International Journal, 16(12): 98-105.