Molecular Cloning, Expression, and Immunogenicity of the sodC Gene of Brucella abortus 544

Author: S. Rajagunalan, Soni Doimari, S. Murugavel and D.K. Singh

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Abstract

Brucellosis is an important zoonotic disease endemic in many parts of the world. Its control and prevention require safe and effective vaccines. Currently available vaccines have certain inherent drawbacks necessitating the need to improve or develop new vaccines. In this study, the sodC gene of Brucella abortus 544, which encodes for Cu-Zn SOD protein, was amplified by polymerase chain reaction (PCR) and cloned in a prokaryotic system. The recombinant protein was induced and purified by nickel affinity chromatography under denaturing conditions, refolded, and used to raise hyperimmune sera in rabbits. Western blot analysis demonstrated a specific reaction between the purified recombinant Cu-Zn SOD and hyperimmune serum, confirming the antigenicity. These results suggested the potential of Cu-Zn SOD as a vaccine candidate.

Keywords

Brucella abortus, sodC gene, Cu-Zn SOD, Cloning, Expression

Conclusion

In conclusion, the sodC gene of Brucella abortus 544 was successfully cloned, expressed, and purified. The recombinant Cu-Zn SOD protein demonstrated promising immunogenicity, supporting its potential as a subunit vaccine candidate.

References

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How to cite this article

S. Rajagunalan, Soni Doimari, S. Murugavel and D.K. Singh (2024). Molecular Cloning, Expression, and Immunogenicity of the sodC Gene of Brucella abortus 544. Biological Forum – An International Journal, 16(1): 337-341.