Molecular Detection of Brucella melitensis: A Case of Sheep Abortion in Tirunelveli, Tamil Nadu

Author: S. Rajagunalan, C. Saranya Vellaiammal, A. Ganesan and A. Sundar

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Abstract

Reproductive loss in farm animals can be caused by various etiological agents investigation of the cause of abortion will help in reducing further loss in the farm. Brucella melitensis is an important zoonotic pathogen causing brucellosis. This disease poses serious economic and public health challenges worldwide. This study investigates a case of sheep abortion that was identified using conventional and molecular detection methods like serological, staining, culture, and PCR-based methods. The causative agent was identified using a bcsp31 gene-based PCR assay and AMOS PCR and is supported by serological tests. The laboratory investigation revealed clear evidence of B. melitensis infection, providing critical insights into the epidemiology of brucellosis in sheep populations in this region. This case underscores the importance of routine molecular diagnostics for early detection of the causative agent and the need for public health interventions to mitigate the impact of this disease.

Keywords

Brucella melitensis, sheep, abortion, AMOS-PCR

Conclusion

This report of B. melitensis-induced abortion highlights the role of brucellosis in reproductive losses among sheep and its public health significance. The findings reinforce the importance of implementing robust diagnostic and control strategies to mitigate the impact of brucellosis on animal health, livelihoods, and public health.

References

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How to cite this article

S. Rajagunalan, C. Saranya Vellaiammal, A. Ganesan and A. Sundar (2024). Molecular Detection of Brucella melitensis: A Case of Sheep Abortion in Tirunelveli, Tamil Nadu. Biological Forum – An International Journal, 16(1): 342-346.