Biosynthesis of Cu Nanoparticles using Leaf Extract of Justicia adhatoda L for their Potent Antimicrobial and Anticancer Activity

Author: Kunj Bhatt, Sanjay Jha, Vaibhav Mehta, Hitesh Mehta, Vasudev Thakkar and Anjali Thakkkar

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Abstract

Nanotechnology is becoming an assurance for scientific advancement nowadays in areas like medicine, consumer products, energy, materials, and manufacturing. Copper nanoparticles (Cu NPs) were synthesized using Ardusi (Justicia adhatoda L) leaf extract via green synthetic pathway. The leaf of Justicia adhatoda L were known to have strong antimicrobial and anticancer properties arising due to the presence of various secondary metabolites, including, flavonoids, alkaloids, saponins, tannins, and phenolic compounds which serve as reducing, stabilizing, and capping agents for the Cu-Nanoparticles (NPs) synthesized. The biosynthesized A CuNPs were characterized based on Dynamic Light Scattering, Zeta- potential, Fourier transform infrared spectroscopy, X-ray diffraction spectroscopy, Scanning electron microscopy, and EDX. Justicia adhatoda L leaf extract mediated synthesis could produce A CuNPs with average hydrodynamic diameter size of 112.0 nm. The biosynthesized A CuNPs were further examined for antibacterial as well as antifungal activity with Gram-positive (S. aureus) and Gram-negative bacteria (E. coli and S. typhi) and with F. oxysporum f. sp. lycopersici respectively. The A CuNPs synthesized using Justicia adhatoda L leaf extract inhibited the growth of S. aureus, E. coli. and S. typhi. Whereas A CuNPs showed considerable inhibition against F. oxysporum f. sp. lycopersici . The A CuNPs showed considerable inhibition against Human lung cancer (A-549) cell line in MTT assay. The newly synthesized nanoparticles were found to be very effective antimicrobial and antifungal agents. Liquid Chromatography-Mass Spectrometry (LC-MS) analysis of Justicia adhatoda leaf extract revealed potent phytochemicals possessing antifungal, antibacterial, and anticancer properties

Keywords

Nanotechnology, Green synthesis, Phytochemicals, Antibacterial activity, Antifungal activity, Anticancer activity, Bioimaging

Conclusion

The eco-friendly synthesis of copper nanoparticles (CuNPs) using medicinal plant like Ardusi harnesses their inherent therapeutic properties, enhancing their effectiveness in combating fungal, bacterial, and cancerous infections. These plants are rich in phytochemicals, which, when incorporated into CuNPs through green synthesis, augment their therapeutic potential. CuNPs inhibit fungal and bacterial growth and exhibit anticancer properties by inhibiting proliferation. Additionally, CuNPs possess unique optical properties, making them promising candidates for cellular imaging. The sustainable synthesis of nanoparticles from medicinal plants presents a viable approach for developing versatile therapeutic agents. Further research into optimization and understanding their mechanisms holds promise for advancing biomedical and pharmaceutical applications, paving the way for innovative therapeutic strategies

References

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How to cite this article

Kunj Bhatt, Sanjay Jha, Vaibhav Mehta, Hitesh Mehta, Vasudev Thakkar and Anjali Thakkkar (2025). Biosynthesis of Cu Nanoparticles using Leaf Extract of Justicia adhatoda L for their Potent Antimicrobial and Anticancer Activity. Biological Forum, 17(3): 12-20