Morphological and Biochemical Characterization of Bacteria Isolated from Sewage Water with Special Reference to Possible Exoelectrogenic Potential

Author: Akanksha S. Jogdand and Rajendra D. Joshi

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Abstract

The research examines bacterial isolation and characterization from sewage water depending on morphological and biochemical attributes. The examination included 157 bacterial isolates that underwent analysis for colony morphology identification together with Gram reaction and biochemical tests. Pseudomonas aeruginosa together with Klebsiella pneumoniae and Escherichia coli made up the majority of bacteria found in sewage microbial communities which belong to the Gram-negative group. Biochemical testing showed that bacteria species which produced positive results in both oxidase and citrate tests commonly occurred in the isolates. This research demonstrates that sewage water contains numerous bacterial communities which have potential uses in wastewater treatment together with biotechnological applications. Research needs to analyze bacterial molecules alongside metabolic measurements to gain comprehensive knowledge about their operational functions

Keywords

Sewage bacteria, morphological characterization, biochemical tests, wastewater microbiota, Pseudomonas aeruginosa, Klebsiella pneumoniae, bacterial diversity

Conclusion

The presence of electricity-generating bacteria within sewage water has been proven through this research with emphasis on their employability for microbial fuel cells. Studies analyzing 157 bacterial isolates that feature Pseudomonas aeruginosa, Klebsiella pneumoniae, Bacillus subtilis and other microorganisms emphasize the role of sewage microbes in creating renewable energy systems. Future research programs need to concentrate on genetic and electrochemical enhancements to improve operational efficiency at practical application sites. Research that covers extended field studies and performs continuous assessments of MFC operational performance will mark the essential transition between laboratory-based knowledge and practical energy solutions utilizing microbial systems. The research determined multiple bacterial types inhabiting sewage water by extracting and characterizing these organisms through microscopic and chemical identification tests. Laboratory work showed the existence of 157 bacterial strains including three major species Pseudomonas aeruginosa, Klebsiella pneumoniae, and Bacillus subtilis which reflects the versatile characteristics of sewage microbial populations. The research results confirm previous studies which emphasized sewage water bacteria as crucial agents in environmental approaches as well as industrial applications. Future investigations must employ 16S rRNA sequencing and related modern molecular identification approaches to achieve exact taxonomic associations of microbial species. Enhanced investigations of metabolic pathways together with studies about enzymatic activities will enable deeper understanding of both ecological functions and industrial possibilities of these bacterial strains The presented study serves as a scientific basis to guide researchers working with sewage microbiota in developing sustainable environmental management practices

References

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How to cite this article

Akanksha S. Jogdand and Rajendra D. Joshi (2023). Morphological and Biochemical Characterization of Bacteria Isolated from Sewage Water with Special Reference to Possible Exoelectrogenic Potential. Biological Forum – An International Journal, 15(3): 959-965