Standardization of Exposure Time of Surface Sterilants on Capitulum Explants of Gerbera (Gerbera jamesonii Bolus ex Hook. f.) cv. Alcatraz for In vitro Culture

Author: Subhadarsini Pradhan, Sashikala Beura, P.N. Jagadev, Pradyumna Tripathy, Biwsajit Mallick, Kaberi Maharana and Subhasmita Sahu

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Abstract

In vitro cloning of gerbera for superior quality, disease free and true-to-type plants has been preferred over conventional vegetative propagation for large scale production of plantlets. The success rate of in vitro cloning is positively correlated with sterilization techniques, sterilants efficacy. Sterilization is the method of making explants contamination free before establishment of culture. An experiment was conducted at Biotechnology-cum –tissue culture centre of OUAT, Bhubaneswar with the objective to establish protocol for standardization of surface sterilization exposure time for capitulum explants of Gerbera hybrid cv. Alcatraz, to obtain culture free from contamination. The MS medium was used as basal medium and supplemented with BAP and IAA was used as culture medium for establishment of explants. Proper sterilisation process was followed for maintaining aseptic environment at in vitro cloning. The explants (capitulum) were exposed to 0.1% HgCl2 for T2 – 2 mins, T3 – 3 mins, T4 – 4 mins, T5 – 5 mins, T6 – 6 mins, T7 – 7 mins and T8 – 8 mins and T1--control (distilled water) inside laminar air flow chamber for surface sterilization. The explants capitulum treated for 6 minutes for surface sterilant of 0.1 % of HgCl2 resulted in minimum fungal (0.00%) and bacterial (0.00%) contamination with maximum survival percentage (90.00%) and minimum death rate (10.00%).

Keywords

Gerbera, Alcatraz, Surface sterilization, HgCl2, In vitro culture

Conclusion

The present study on standardization of surface sterilization exposure time on capitulum of Gerbera hybrid cv. Alcatraz concluded that there is a positive correlation between sterilant efficacy and success rate of in vitro cloning. The explants capitulum treated for 6 minutes for surface sterilant of 0.1 % of HgCl2 resulted in minimum fungal (0.00%) and bacterial (0.00%) contamination with maximum survival percentage (90.00%) and minimum death rate (10.00%).

References

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How to cite this article

Subhadarsini Pradhan, Sashikala Beura, P.N. Jagadev, Pradyumna Tripathy, Biwsajit Mallick, Kaberi Maharana and Subhasmita Sahu (2022). Standardization of Exposure Time of Surface Sterilants on Capitulum Explants of Gerbera (Gerbera jamesonii Bolus ex Hook. f.) cv. Alcatraz for In vitro Culture. Biological Forum – An International Journal, 14(5): 22-25.