Studies on Effectiveness and Efficiency of Gamma Rays, Ethyl Methane Sulphonate and Sodium Azide in Mungbean (Vigna radiata (L.) Wilczek)

Author: S. Sofia, D. M. Reddy, K.H.P. Reddy, P. Latha, B. Ravindra Reddy and M. Sreevalli Devi

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Abstract

In the present study, dry seeds of two mungbean varieties viz., WGG-42 and LGG-460 were treated with different doses/ concentrations of gamma rays (200 Gy, 300 Gy, 400 Gy, 500 Gy and 600 Gy), ethyl methane sulphonate (0.2%, 0.3%, 0.4%, 0.5% and 0.6%) and sodium azide (1 mM, 2 mM and 3 mM) to study the mutagenic effectiveness and efficiency of gamma rays, ethyl methane sulphonate and sodium azide in induction of chlorophyll mutations in M2 generation. Three types of chlorophyll mutants viz., albina, xantha and chlorina were observed in M2 generation and occurrence of chlorina was found to be the most frequent in all the mutagen treated populations of both the varieties. The frequency of chlorophyll mutations increased with the increase in dose/concentration of mutagens in both the genotypes, but EMS treatments showed the highest frequency of chlorophyll mutations than gamma rays and SA treatments. The results indicated that the values of mutagenic effectiveness and efficiency gradually decreased with increases in dose/concentration of mutagens. The EMS treatments were found to be more effective and efficient in inducing chlorophyll mutants than gamma rays and sodium azide in both the varieties. Thus, it is concluded that the lower doses/concentrations of mutagens are more useful to induce different type of chlorophyll mutants in mungben.

Keywords

Gamma rays, EMS, SA, chlorophyll mutants, effectiveness and efficiency, M2, mungbean

Conclusion

From the results of the present study, it can be inferred that the frequency of chlorophyll mutations increased with the increase in dose/concentration of mutagens in both the genotypes, but EMS treatments showed the highest frequency of chlorophyll mutations than gamma rays and SA treatments. Mutagenic effectiveness and efficiency gradually decreased with increases in dose/concentration of mutagens. It was also inferred from the present study, EMS treatments were found to be more effective and efficient in inducing chlorophyll mutants than gamma rays and SA treatments in both the varieties viz., WGG-42 and LGG-460. These results suggest that high mutation rates could be obtained with lower/moderate dose/concentration of mutagens in mungbean.

References

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How to cite this article

S. Sofia, D. M. Reddy, K.H.P. Reddy, P. Latha, B. Ravindra Reddy and M. Sreevalli Devi (2023). Studies on Effectiveness and Efficiency of Gamma Rays, Ethyl Methane Sulphonate and Sodium Azide in Mungbean (Vigna radiata (L.) Wilczek). Biological Forum – An International Journal, 15(10): 1299-1304.