Groundnut (Arachis hypogaea L.), a major food grain legumes is an important economic crop that yields vegetable oil and serves as a rich protein source. Crop improvement through modern biotechnological approaches necessitates establishment of cell cultures for multiple uses. Groundnut like other legumes are recalcitrant to tissue culture and any attempt in optimizing protocol for cell cultures is considered to be a boon for plant genetic manipulation. The present study was conducted to evaluate various factors influencing callus initiation for subsequent cell cultures establishment from various explants under in vitro condition in an elite groundnut cultivar TMV (Gn) 13. Leaf and DEC explants are considered as the best choice of explants for callus induction in TMV (Gn) 13 with callus induction frequency of 97.22% when cultured in MS medium supplemented with 5.0 mg l -1 2, 4-D. The effect of 2, 4-D alone to stimulate better callus induction in various explants of groundnut is demonstr

Auxin, callus, cell cultures, Arachis, Groundnut, tissue culture.

The present study has made efforts to optimize protocol for callus induction in groundnut. Leaf and DEC explants are considered as the best choice of explants for callus induction in TMV (Gn) 13 with callus induction frequency of 97.22 % when cultured in the MS medium supplemented with 5.0 mg l-1 2,4-D. The effect of 2,4-D alone to stimulate better callus induction in various explants of groundnut is demonstrated. Further, in vitro studies through indirect organogenesis can be attempted with various explants and hormonal combinations for successful plant regeneration in groundnut.

Mathew, F.A., Rajesh, S., Rajagopal, B. and Chandirakala, R. (2021). Studies on Factors influencing callus Induction in elite Groundnut (Arachis hypogaea) cultivar TMV (Gn) 13. Biological Forum – An International Journal, 13(2): 540-545.