Studies on Green Synthesis of Silver Nanoparticles using Aqueous Leaf extract of Sauropus androgynus and their Antibacterial Activity

Author: Krishna Prasanth Y., Vijayakumar R.M., Madhumitha B., Jenita Thinakaran and Umesh Chimmalagi

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Abstract

The synthesis of nanoparticles using environmentally friendly methods has attracted significant interest owing to their versatile applications and the need for sustainable approaches. Silver nanoparticles (SNPs) have emerged as a popular choice owing to their unique properties; however, they have drawbacks due to the involvement of hazardous chemicals. This study contributes to the field of environmentally friendly nanoparticle synthesis using Sauropus androgynus leaf extract as a natural reducing agent. This approach eliminates the use of hazardous chemicals that are typically involved in traditional synthesis methods, making it a more sustainable alternative. SEM, zeta potential analysis, UV-visible spectroscopy, and X-ray diffraction (XRD) were used to characterize the synthesized nanoparticles. The absorption peak at 420 nm in the UV-visible spectrum is evidence of the presence of SNPs. Spherical nanoparticles with an average size of 219 nm were discovered using SEM. An examination of zeta potentials revealed a negative zeta potential of -11.1 mV, demonstrating the stability of the nanoparticles. The crystalline nature of the nanoparticles was confirmed by X-ray diffraction (XRD) measurements, with an average crystalline size of 16.23 nm. The antibacterial activity of the synthesized nanoparticles was evaluated against both gram-positive (Bacillus subtilis) and gram-negative (Escherichia coli) bacteria at different concentrations. The results showed that Sauropus androgynus leaf extract-mediated SNP had antibacterial activity, and the extent of inhibition zones was concentration-dependent. This study addresses the challenges associated with green synthesis of SNPs and provides valuable insights into their characterization and antibacterial activity.

Keywords

Sauropus androgynus, Bacillus subtilis, Escherichia coli, silver nanoparticles

Conclusion

In conclusion, this study successfully demonstrated the environmentally friendly synthesis of silver nanoparticles (SNPs) using Sauropus androgynus leaf extract as a natural reducing and capping agent. The synthesized nanoparticles exhibited favorable physicochemical properties, including size, shape, stability, and crystallinity, which are crucial for their potential applications in various fields. Furthermore, the antibacterial activity of the silver nanoparticles was evaluated against two common bacterial strains, namely B. subtilis and E. coli. The results revealed significant inhibitory effects, indicating the potential of these nanoparticles for antimicrobial applications. The inhibition zones observed were larger at higher concentrations, suggesting a concentration-dependent antibacterial activity. Interestingly, the silver nanoparticles exhibited more pronounced activity against E. coli compared to B. subtilis. These findings underscore the promising prospects of utilizing Sauropus androgynus leaf extract-mediated silver nanoparticles as an effective antimicrobial agent. The green synthesis approach utilized in this study provides a sustainable and eco-friendly alternative to conventional methods involving hazardous chemicals.

References

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How to cite this article

Krishna Prasanth Y., Vijayakumar R.M., Madhumitha B., Jenita Thinakaran and Umesh Chimmalagi (2023). Studies on Green Synthesis of Silver Nanoparticles using Aqueous Leaf extract of Sauropus androgynus and their Antibacterial Activity. Biological Forum – An International Journal, 15(5): 755-759.