Studies on Lipoxygenase activity levels in seed and during seed germination in different genotypes of tobacco (Nicotiana tabacum L.)

Author: K. N. Subrahmanya* and M. Krishnappa**

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Abstract

Lipoxygenase activity was measured in seed and germinating seeds of different genotypes of tobacco with varied reaction to the pathogen Tobacco Mosaic Virus. Lipoxygenase enzyme activity level in seeds of resistant genotype Va 770 was double than that in the susceptible (FCV Spl.). The trend of results was validated with a series of genotypes with varied levels of resistance/susceptibility. The susceptible genotypes recorded the lowest activity. The level of enzyme during sprouting in tobacco mosaic virus resistant genotype was 60% higher as compared to that in susceptible variety. The tolerant variety showed only 20% higher activity than the susceptible. Higher lipoxygenase activity may have a protective function in resistant genotype at the initial stage of germination (sprout) which is vulnerable to infection. Extent of activation of enzyme during seed germination and seedling growth varied with the variety. Initial activation of lipoxygenase followed by a decrease in enzyme activit

Keywords

Lipoxygenase, Enzyme, Seed, Tobacco Mosaic Virus, Resistance, Tobacco

Conclusion

Lipoxygenase activity was measured in seed and germinating seeds of different genotypes of tobacco with varied reaction to the pathogen Tobacco Mosaic Virus. Lipoxygenase enzyme activity level in seeds of resistant genotype Va 770 was double than that in the susceptible (FCV Spl.). The trend of results was validated with a series of genotypes with varied levels of resistance/susceptibility. The susceptible genotypes recorded the lowest activity. The level of enzyme during sprouting in tobacco mosaic virus resistant genotype was 60% higher as compared to that in susceptible variety. The tolerant variety showed only 20% higher activity than the susceptible. Higher lipoxygenase activity may have a protective function in resistant genotype at the initial stage of germination (sprout) which is vulnerable to infection. Extent of activation of enzyme during seed germination and seedling growth varied with the variety. Initial activation of lipoxygenase followed by a decrease in enzyme activit

References

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How to cite this article

K. N. Subrahmanya* and M. Krishnappa (2017). Studies on Lipoxygenase activity levels in seed and during seed germination in different genotypes of tobacco (Nicotiana tabacum L.) , Biological Forum – An International Journal 9(1): 10-13.