Studies on the Investigation of Anti-Oxidant and Cellular Toxicity on L929 Cell Line of Andrographis paniculata and its Derivative of Silver and Gold Nanoparticles

Author: M. Shirley Navis and Ragel Mabel Saroja

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Abstract

The current pharmaceutical industry is increasingly turning to plant-based multi-potential bioactive compounds to combat a variety of diseases and pathological conditions due to the consequences of administrating synthetic drugs. To Avoid back reflection of synthetic drugs, researchers are focusing on natural derivatives guided from traditional medical practices. Plants are playing vital role in nutritional and disease control aspects in traditional era. Terrestrial resources contain a vast array of medicinally valuable plants that have traditionally been used to treat a wide range of diseases, including hepatoprotective, anti-inflammatory, antibacterial, anti-cancer, and antidiabetic properties. Similarly, the study plant Andrographis paniculata demonstrated significant medicinal properties; thus, the potential activity of a leaf ethanolic extract of A. paniculata, diethyl phthalate purified from the crude ethanolic extract, and their modified metal (Silver and Gold) nanoparticles against free radicals, namely DPPH and phosphomolebdenum assays. In addition, the study samples were subjected to an MTT assay to assess cellular toxicity in comparison to the L929 cell line. The results showed that the promising drug carrier system of diethyl phthalate silver nanoparticles (DPAgNPs) exhibited lower toxicity and higher anti-oxidant activity.

Keywords

Andrographis paniculata, Diethyl phthalate, Silver and gold nanoparticles, DPPH, Phospohomolebdenum and Cytotoxicity

Conclusion

Nowadays, modern pharmacologists have turned to traditional and classic medicinal approaches for deriving multi-potential bioactive compounds to encounter a wide range of diseases and physiological disorders. In this research, the anti-oxidant potential and cytotoxic effects of a crude ethanolic extract of Andrographis paniculata, diethyl phthalate, silver, and gold nanoparticles synthesised using diethyl phthalate were evaluated, and the outcome revealed the improved anti-oxidant activity achieved from DPAgNPs compared with other test samples against both radicals (DPPH and phosphomolebdenum). It was also demonstrated that DPAgNPs had a less toxic effect on the L929 cell line among the study samples. The data show that using DPAgNPs for developing drug carrier systems is far superior to using A. paniculata, diethyl phthalate, and DPAuNPs alone, resulting in safe and fine activities.

References

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How to cite this article

M. Shirley Navis and Ragel Mabel Saroja (2023). Studies on the Investigation of Anti-Oxidant and Cellular Toxicity on L929 Cell Line of Andrographis Paniculata and its Derivative of Silver and Gold Nanoparticles. Biological Forum – An International Journal, 15(4): 590-597.