Study of Seed Protein Profiling of Cucumber (Cucumis sativus L.) Genotype using SDS-PAGE for Determination of Genetic Relationship

Author: Shashank Shekhar Singh* and Khushboo Kathayat

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Abstract

A quantitative categorization of seed storage protein profile of 25 genotypes of Cucumis sativus L. was performed by Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE). The banding patterns were characterized by 3 clear district zones i.e. Zone A, Zone B and Zone C. Zone A has 9 bands, zone B has 10 bands and zone C included 8 bands. Maximum number of bands were observed in NDCUC-106 (9 bands) followed by NDCUC-160 (8 bands) whereas, minimum bands were shown by PPC-2, PPC-3, Pant Khira-1 and NDCUC-73 i.e. 1bands. The unweight pair group method using arithmetic average (UPGMA) analysis of 25 cucumber genotype was done and two major cluster obtained through seed protein analysis expressed better grouping of genotypes.

Keywords

Cluster analysis, Germplasm, SDS-PAGE, Cucumis sativus L., Cucumber.

Conclusion

Protein profiling is an effective method for identification of genetic diversity, combining biochemical approaches with molecular approaches would help plant breeder to understand the diversity/ variance among plants so it is concluded from the study that NDCUC-16 and NDCUC-16 and NDCUC-14 are most diverse among all genotypes and may be further utilized as potential source in cucumber breeding programme for the successful varietal development.

References

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How to cite this article

Shashank Shekhar Singh and Khushboo Kathayat (2022). Study of Seed Protein Profiling of Cucumber (Cucumis sativus L.) Genotype using SDS-PAGE for Determination of Genetic Relationship. Biological Forum – An International Journal, 14(1): 627-631.