Study on the Evaluation of Toxicity of Nerium indicum (M.) Against Cabbage Butterfly, Pieris brassicae (L.) (Lepidoptera: Pieridae)

Author: Summira Rafiq*, R.S. Bandral, Suheel Ahmad Ganai, Rehana Akbar, Shafiya Rashid and Roohi Jan

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Abstract

Plant extracts contain many active compounds, which are tremendously fruitful for plant defence against several insect pests. This study is aimed to evaluate the leaf and bark extracts of Nerium indicum plant against cabbage butterfly, Pieris brassicae. The toxicity activity of leaf and bark extracts of N. indicum M. against second instar larvae of cabbage butterfly was carried out using methanol, hexane and distilled water as solvents by no choice method. Different concentrations of leaf and bark (100, 75, 50, 25 and 10%) were used in this study. The results revealed that among various solvents, methanol found to be significantly superior in extracting the toxic constituents than the hexane and distilled water. The highest concentration (100%) of methanol leaf extract used in this study, showed mortality of 40.0, 46.7 and 53.3 per cent after 24, 48 and 72 hours of exposure, respectively. whereas, at same concentration and time period, hexane and distilled water leaf extract showed 33

Keywords

Nerium indicum, leaf and bark extracts, cabbage butterfly, Per cent mortality.

Conclusion

The results revealed that the per cent mortality in N. indicum leaf and bark extracts showed significant effect against second instar larvae of cabbage butterfly which were increased with the increase in the concentrations of extracts and time period. The results indicate that mortality values significantly increased depending on the increasing leaf and bark extract concentrations and exposure time. Among leaf extracts, methanol leaf extract at 100% concentration was the best treatment which recorded highest per cent mortality of 40.00, 46.67 and 53.33 after 24, 48 and 72 hours of treatment. However among bark extracts, again methanol bark extract proved as most effective which resulted in mortality of 31.67, 35.00 and 36.67% at respective concentrations. Maximum cumulative mortality of 53.33 and 36.67% after 72 hours of exposure was recorded at the highest concentration (100%) for methanol leaf and bark extracts of N. indicum. Whereas, minimum cumulative mortality of 28.33 and 25.00%

References

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How to cite this article

Summira Rafiq, R.S. Bandral, Suheel Ahmad Ganai, Rehana Akbar, Shafiya Rashid and Roohi Jan (2022). Study on the Evaluation of Toxicity of Nerium indicum (M.) Against Cabbage Butterfly, Pieris brassicae (L.) (Lepidoptera: Pieridae). Biological Forum –