Surface Sterilization and in vitro Callusing of Gerbera jamesonii Bolus cv. Balance

Author: Ruchi Sharma and Sashikala Beura

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Abstract

Microbial contamination is one of the major setback and act as a barrier in establishing in vitro cultures for which the disinfection procedure of explants prior to inoculation is important and this experiment was investigated with the objective to procure disease free in vitro aseptic culture of Gerbera jamesonii Bolus cv. Balance and callus induction in supplemented MS media using quarter capitulum as explant. Explants for surface sterilization treatment were subjected to 0.1% HgCl2 solution alone for different span of time and in combination with 0.5% NaOCl solution for 2 minutes along with control (Distl. water) and result revealed significant reduced rate of fungal and bacterial contamination with increased aseptic cultures when explant were soaked with 0.1% HgCl2 solution for 6 min. followed by 0.5% NaOCl solution for 2 minutes. The aseptic culture obtained were transferred in MS media containing different combinations of BAP, IAA for callus induction and callus proliferation, wh

Keywords

gerbera, in-vitro, capitulum, surface sterilization, callus