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Synergistic Effect of Putrescine and TDZ on in vitro Regeneration of Lagerstroemia speciosa L.

Author: Waquar Ahmad and Saad Bin Javed

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Abstract

Lagerstroemia speciosa, an important medicinal plant known for its anti-diabetic properties due to the presence of Corosolic acid, presents challenges in commercial cultivation due to its long life cycle as a tree. This study aimed to overcome these challenges by developing a highly reproducible protocol for in vitro regeneration of L. speciosa using nodal segments or shoot tips. Various concentrations of TDZ were tested for in vitro shoot bud induction, followed by transfer to WPM media supplemented with cytokinin. The study found that the combination of putrescine (40µM) and mT (5 µM) in the WPM medium yielded the highest number of shoots (18.6) with an average shoot length of 13.8cm in 87.2% of the cultures after 8 weeks of incubation. Successful rhizogenesis was induced in shootlets using ½ WPM supplemented with indole-3-butyric acid (0.5 µM). Finally, the regenerated plantlets with well-developed roots were successfully acclimatized.

Keywords

Regeneration, TDZ, meta-Topolin, Putrescine

Conclusion

An efficient protocol for in vitro regeneration has been developed to enable mass propagation of Lagerstroemia speciosa L. using shoot tips or nodal segments as explants. The study also highlights the stimulating effect of putrescine on the in vitro regeneration potential of L. speciosa induced by TDZ. The highest induction of shoot buds was achieved on WPM medium supplemented with mT (5µM) + put (40 µM), exceeding the effects of BA and KIN. Additionally, IBA (0.5µM) was found to be the most effective hormone for in vitro rooting, resulting in an average of 4.5 roots with a mean length of 4.9cm in 86.8% of cultures. The regenerated plantlets, exhibiting well-developed roots, were successfully acclimatized and transferred to field conditions.

References

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How to cite this article

Waquar Ahmad and Saad Bin Javed (2023). Synergistic Effect of Putrescine and TDZ on in vitro Regeneration of Lagerstroemia speciosa L. Biological Forum – An International Journal, 15(5): 607-613.