Synergistic Interaction of Probiotics in Multispecies Filtrate Form against Food Borne Pathogens

Author: Anita Raisagar and Sangeeta Shukla

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Abstract

Previous studies suggested that the multi-strain probiotic preparation exhibits better inhibitory activity against food borne pathogens as compared to single strain probiotic preparation. Thus, in the present study, two different probiotic bacteria were prepared as multi-species filtrate form and evaluated for their antibacterial properties against selected four food borne pathogens. Lacticaseibacillus casei and Bifidobacterium bifidum was selected as probiotics in the present study. Against all the pathogens, multispecies probiotic filtrate showed high inhibition capacity. The recorded zone of inhibition was found greater with multispecies probiotic filtrate as compared to mono-strain form. In calculation of GIIs, the interaction was found synergistic between the selected probiotics. Thus, probiotics in mixed form might be useful for treatment of food borne diseases.

Keywords

Bifidobacterium bifidum, Food borne pathogens, Lacticaseibacillus casei, Multispecies, Mono-strain, Probiotic Filtrate, Synergistic effect

Conclusion

In the present study, the selected probiotic bacteria Lacticaseibacillus casei and Bifidobacterium bifidum possess antibacterial activity against selected food borne pathogens and showed synergistic interaction in combined form as multi-species probiotic filtrate. Multi-species probiotic filtrate showed higher zone of inhibition against pathogens as compared to mono-strain form. Thus, the study also concluded that to get a better result in food borne disease prevention, probiotic bacteria would be used as multi-species filtrate form. However, further study is required to check the efficiency of selected probiotic culture in multi-species filtrate form against another enteric and food borne pathogens. There is also a need of in vivo study of effect of multi-species probiotic filtrate against tested food borne pathogens.

References

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How to cite this article

Anita Raisagar and Sangeeta Shukla (2023). Synergistic Interaction of Probiotics in Multispecies Filtrate Form against Food Borne Pathogens. Biological Forum – An International Journal, 15(5): 1455-1459.