The Effect of 6-Benzylaminopurine on hyp-1gene in Hypericum perforatum L.

Author: Zeynab Nasripour*, Nader Farsad Akhtar*, Nasser Mahna**, Rouhollah Motafakkerazad* and Parisa Benamolaei***

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Abstract

Considering the importance of hyp-1 gene function in hypericin biosynthesis in Hypericum perforatum, we studied the effect of various concentrations of benzylaminopurine (BAP) on the expression of this gene. In this way, calli from leaf explants were treated with three concentrations of BAP and the activity of hyp-1 gene was evaluated via Real Time PCR, 24, 48 and 72 hours after treatment. In control samples there was no variation in gene expression. In the samples treated with 0.5 mg/l BAP, 72 hours after treatment, the expression of hyp-1 gene was significantly increased. In the samples treated with 2 mg/l BAP, a 3-fold increase was resulted in the expression of hyp-1 gene in all sampling times in comparison with control. In this project, the minimum hyp-1 gene expression was after 24 hours in control while the maximum was witnessed in 72 hours after treatment with 2 mg/l BAP.

Keywords

BAP, Gene expression, hyp-1 gene, Hypericin, Hypericum perforatum,

Conclusion

Considering the importance of hyp-1 gene function in hypericin biosynthesis in Hypericum perforatum, we studied the effect of various concentrations of benzylaminopurine (BAP) on the expression of this gene. In this way, calli from leaf explants were treated with three concentrations of BAP and the activity of hyp-1 gene was evaluated via Real Time PCR, 24, 48 and 72 hours after treatment. In control samples there was no variation in gene expression. In the samples treated with 0.5 mg/l BAP, 72 hours after treatment, the expression of hyp-1 gene was significantly increased. In the samples treated with 2 mg/l BAP, a 3-fold increase was resulted in the expression of hyp-1 gene in all sampling times in comparison with control. In this project, the minimum hyp-1 gene expression was after 24 hours in control while the maximum was witnessed in 72 hours after treatment with 2 mg/l BAP.

References

St. John's wort (Hypericum perforatum L.) is an important medicinal plant due to having secondary metabolites like hypericin, pseudohypercin and hyperforin (Figuerio et al, 2010). These and other metabolites, despite having many physiological functions in plant cells during its lifecycle, serve as the best sources for phytopharmaceaticals (Kosuth et al, 2007). Staggering amount of work has been performed to produce more metabolites from in and ex vitroplant materials (Vardapetyan et al. 2006). To increase the efficiency of metabolite production in cell cultures, culture condition improvement and application of elicitors have been used (Kirakosyan et al, 2001). These conditions include culture medium salts, vitamins, amount of sugar, temperature, light intensity and quality, organic compounds, hormones and so forth. At the moment, the majority of medicinal plants genome is known and the communications among enzymes, internal and external signals and other molecules is yet to be cleare

How to cite this article

Zeynab Nasripour, Nader Farsad Akhtar, Nasser Mahna, Rouhollah Motafakkerazad and Parisa Benamolaei (2017). The Effect of 6-Benzylaminopurine on hyp-1gene in Hypericum perforatum L. Biological Forum – An International Journal 9(1): 145-149.