The efficacy of Ascorbic acid against Lead Nitrate Impact on the Histology of Liver of Common Carp (Cyprinus carpio)

Author: Musrat Majeed, Rajendra Chauhan, Farooq Ahmad Mir and Shaista Masarat

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Abstract

Lead is reported as heavy metal that induces physiological dysfunction and blood disorders. They are often firmly attached to polypeptides and proteins and are water soluble but non-degradable. Diverse activities continue to have an impact on the aquatic environment, altering the climate and causing health hazards to fish, despite past study issuing cautionary notes. This study assessed the toxicity of lead nitrate to the freshwater fishes Cyprinus carpio, to determine the hematological toxicity, histopathology and mortality and survival rate. Fishes were exposed to sub lethal lead nitrate concentrations 5ppm for 15 and 30 days of exposure and (percentage)% mortality was noted after 24, 48, 72, and 96 hours. The determination of LC50 of the Lead nitrate during the present experimental period. 10 mg/l to 60 mg/l Lead nitrate was used to assess the rate of mortality at each concentration. 60 mg/l was seen to be LC100 for Lead nitrate. 50% mortality was recorded in experimental groups exposed to 30-40 mg/l of Lead nitrate therefore, the acute 96h LC50 value for the present experimental fish, Common carp was calculated to be 35 mg/l (ppm).In the present research, the significant hematological (WBCs, RBCs, Hb, neutrophils, Basophils MCHC and Lymphocytes) and histopathological alteration in liver tissue of common carp was recorded upon the exposure to different concentration of lead. The results concluded that lead has a strong influence on hematological and histopathological parameters of common carp during chronic toxicity.

Keywords

Lead nitrate, C.carpio, mortality, probit, LC50, dysfunction

Conclusion

The impacts of heavy metals on fish are more challenging to visualize because of the aquatic ecosystem's dynamic nature. Similar research was performed to determine the amount of lead present in water and it affects natural populations of freshwater fish by altering their physiology, abilities, and skills. The results have significant implications for ecological risk assessments and could make it difficult for fish to survive in their natural habitat. Since consumption of fish food containing heavy metals can have an impact on human health by spreading through the food chain, there is a constant need to identify heavy metal toxicity. Long-term exposure to the genotoxicity that occurred in common carp might have a major negative impact on the health of fish.

References

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How to cite this article

Musrat Majeed, Rajendra Chauhan, Farooq Ahmad Mir and Shaista Masarat (2023). The efficacy of Ascorbic acid against Lead Nitrate Impact on the Histology of Liver of Common Carp (Cyprinus carpio). Biological Forum – An International Journal, 15(5): 1674-1680.