Whitefly (Bemisia tabaci) and mustard aphid (Lipaphis erysimi L.) are the most destructive insect pests infesting okra (Abelmoschus esculentus) and mustard (Brassica juncea), respectively, leading to substantial yield losses and compromising yield and oil quality of the crop. This study evaluates the field efficacy of the entomopathogenic fungus Lecanicillium lecanii and other bio-insecticides as environmentally sustainable alternatives to conventional chemical pesticides. Field trials were conducted across one cropping seasons to assess the impact of L. lecanii, neem-based formulations and other biorational products on pest incidence, population dynamics and crop yield. Results revealed that L. lecanii significantly reduced whitefly and aphid populations compared to untreated controls. Imidacloprid 17.8% SL were found superior treatment reduced the white fly and aphid population (80% and 85.08%) among the EPF and their combination treatment T6 – L. lecanii 10% concentration observed best treatment to reduced the population 75% and 76.18% at 10th DAS for broth the insect, respectively. EPF showing comparable effectiveness to standard chemical treatments but with added benefits of environmental safety and compatibility with integrated pest management (IPM) strategies. The study underscores the potential of L. lecanii and similar bio-insecticides as viable tools in sustainable pest management programs for okra and mustard cultivation. Further exploration into formulation improvements and application timing may enhance efficacy and farmer adoption

Lecanicillium lecanii, whitefly, aphid, bio-insecticides, field efficacy, integrated pest management

On the basis of present study we concluded that Imidacloprid 17.8% SL were found superior treatment to reduced the white fly and aphid population (80% and 85.08%), respectively and among the EPF and their combination treatment T6 – L. lecanii 10% concentration observed best treatment to reduced the population 75% and 76.18% at 10th DAS for whitefly and mustard aphid, respectively

Anjali Shandilya, Vinod Kumar Nirmalkar, R.K.S. Tiwari and Yaspal Singh Nirala (2025). To Evaluate the Potential Isolates of L. lecanii Against Whitefly (Bemisia tabaci) and Aphid (Lipaphis erysimi) under Natural Field Condition. Biological Forum, 17(5): 134-139