To Test the Residual Toxicity of the Selective Nicotinic Receptor Antagonist- Spinosad on the Callosobruchus chinensis L. pulse Beetle (Coleoptera: Chrysomelidae) in a Lab Setting

Author: A.N. Warghat, A.R. Tayde and S.S. Munje

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Abstract

The study was conducted in the Department of Entomology lab at the AICRP (Soybean)- RRC, Amravati Dr. P.D.K.V., Akola (Maharashtra State), Regional Research Centre for (Soybean) in Akola between 2020 and 2021. The study's findings, To Test the Residual Toxicity of the Selective Nicotinic Receptor antagonist-Spinosad on the Callosobruchus chinensis L. pulse beetle (Coleoptera: Chrysomelidae) in a Lab Setting, were well-detailed, and it was evident that the study of rearing insects of the pulse beetle, Callosobruchus chinensis L. The goal of the laboratory tests was to create a homogeneous population. From there, Spinosad 45% SC relative toxicity against the pulse beetle, C. chinensis, was assessed based on the percentage of adult mortality of resistant species classified as Susceptible (S5) and Resistant Population (R1) and count the probit analysis of Susceptible (S6) and Resistant Population (R2). The bioassay, which was carried out using the impregnated filter paper method with five concentrations of each of the four treatments, including the control, and reproduced three times with Spinosad 45% SC, revealed that the higher concentrations of Spinosad had greater toxicity than the lower doses and Mortality percentage was noticed right away at 24 HAT in a 30 ppm concentration, where the average mortality was (8.33%), followed by the next treatment at 50 ppm, which was (10.00%), followed by 70 ppm, which showed that (13.33%), next to 90 ppm, which showed (15.00%), next to 150 ppm, which showed (16.67%) mortality, respectively. The average mortality was 8.33% at 48 HAT in a 30 ppm concentration, followed by 50 ppm treatment, or (11.67%), then 70 ppm revealed that (15.00%), then 90 ppm showed (18.33%), and finally 150 ppm showed (21.67%). Mortality was discovered to be 1.67 times higher than the water spray control. The average mortality at 72 HAT 30 ppm concentration was (35.00%), followed by the next treatment at 50 ppm, which was (38.33%), then 70 ppm showed that (45.00%), then 90 ppm showed (50.00%), and finally 150 ppm showed (53.33%), respectively. Mortality was also found when compared to the control (water spray), which was (8.33) found least frequently. The Probit analysis of the mortality % and extract concentration at 24, 48, and 72 hours after treatment revealed a linear connection when probit regression lines of the Spinosad resistant (S6) and (R2) populations were generated. According to the analysis, the regression line equations for (T5) 150 ppm were Y = 0.773x + 3.219 24 hours after treatment. The tested pesticides' computed X2 values were lower than the table value (0.951), indicating that the adult population was homogenous. The regression line equations were computed as Y= 0.979x + 2.067 for (T5) 150 ppm at 48 hours post-treatment. The tested pesticides computed X2 values were lower than the table value (0.951), indicating that the adult population was homogenous. The regression line equations for (T5) 150 ppm at 72 HAT were derived as Y= 0.773x + 3.221. The tested pesticides' computed X2 values were lower than the table value (0.951), indicating that the adult population was homogenous. This study suggests that eco-friendly management can help break down pest resistance in food grains, allowing them to be stored for longer periods without contamination.

Keywords

Resistant species, population, stored grain pest, probit analysis, residual toxicity, Spinosad, Agro chemical

Conclusion

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How to cite this article

A.N. Warghat, A.R. Tayde and S.S. Munje (2023). To Test the Residual Toxicity of the Selective Nicotinic Receptor Antagonist- Spinosad on the Callosobruchus chinensis L. pulse Beetle (Coleoptera: Chrysomelidae) in a Lab Setting. Biological Forum – An International Journal, 15(10): 137-142.