Use of Microsatellite Markers for Genetic Diversity Analysis of Olive Germplasm in the North of Iran
Author: Ali Bahmani*, Mohammad-Reza Dadpour*, Asad Asadi-Abkenar**, Fariborz Zare-Nahandi*
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Abstract
Olive (Olea europaea L.) is one of the subtropical fruit tree with remarkable economic importance. This study was conducted to evaluate the variation and inter-relationship of 31 olive genotypes including seven Iranian and 24 foreign genotypes collected in a germplasm using Simple Sequence Repeat (SSR) markers. In this research, 7 SSR primer pairs out of 17 SSRs were used for fingerprinting of the genotypes. Totally, 37 SSR polymorphic alleles were detected. Classification of genotypes based on UPGMA criterion located them in three major groups. In this study, Iranian cultivars and ecotypes of olive, clustered separately from Mediterranean cultivars. This is concluded that SSR markers could efficiently clarify the existent genetic variability in olive, and the identified genetic variability is somewhat in coincidence with the geographical distribution of olive genotypes.
Keywords
Olive, Simple sequence repeat, similarity, cluster analysis
Conclusion
Olive (Olea europaea L.) is one of the subtropical fruit tree with remarkable economic importance. This study was conducted to evaluate the variation and inter-relationship of 31 olive genotypes including seven Iranian and 24 foreign genotypes collected in a germplasm using Simple Sequence Repeat (SSR) markers. In this research, 7 SSR primer pairs out of 17 SSRs were used for fingerprinting of the genotypes. Totally, 37 SSR polymorphic alleles were detected. Classification of genotypes based on UPGMA criterion located them in three major groups. In this study, Iranian cultivars and ecotypes of olive, clustered separately from Mediterranean cultivars. This is concluded that SSR markers could efficiently clarify the existent genetic variability in olive, and the identified genetic variability is somewhat in coincidence with the geographical distribution of olive genotypes
References
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How to cite this article
Ali Bahmani, Mohammad-Reza Dadpour, Asad Asadi-Abkenar, Fariborz Zare-Nahandi (2016). Use of Microsatellite Markers for Genetic Diversity Analysis of Olive Germplasm in the North of Iran Biological Forum – An International Journal 8(1): 27-31