North East India has rich diversity of rice’s, among them three land races of black aromatic glutinous rice of Manipur i.e. Chakhao Poireiton, Black Chakhao and Chakhao Amubi have gained a lot of importance in domestic as well as International market. This black rice’s are poor yielders and are susceptible to major biotic and abiotic stresses. In the present investigation, three black rice land races i.e., Chakhao Poireiton, Black Chakhao and Chakhao Amubi were screened for blast and bacterial blight genes. To determine the presence of any blast and blight resistance genes, phenotyping on UBN using fungal isolates for blast, clip clipping with blb inoculum for blight in field was done and genotyping for two major blast (Pi54 and Pi2) resistant genes and three major blight (Xa21, xa13 and xa5) resistant genes were carried out by using functional markers (RM206, AP5659-5, pTA248, xa13 promoter and xa5FP, respectively). Both phenotyping and genotyping results have shown that black ric

Black rice, Chakhao Poireiton, Black Chakhao, Chakhao Amubi, blast resistance, bacterial leaf blight resistance, marker-assisted selection.

As the domestic demand for the black glutinous aromatic rice of Manipur is increasing, efforts are needed to improve the existing landraces by introgressing major blast and BLB resistant genes through molecular breeding approach which increases their yield without losing the grain's quality, cooking quality and aroma and encouraging the farmers to cultivate black rice. To the best of our knowledge, there was no early work done on blast and bacterial blight in black rice varieties. The identified polymorphic foreground markers from the present study will be utilized for genotyping the backcross and intercross population possessing blast and blight genes.

Noor Ahmed, S., Madhav, M.S., Padma, V. and Satish, Y. (2021). Validation of Linked Markers of Blast and Blight Resistance Genes among Black Rice Accession’s of North East India. Biological Forum – An International Journal, 13(2): 293-297.