Wound Healing Gauze Formulation through Synthesis of Selenium Nanoparticles from Sericin and Wrightia tinctoria – A Green Approach

Author: M. Sridevi, R. Siva Renjith, A. Surya and C. Nirmala

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Abstract

Wound provides a environment for microbial growth which delays healing and cause more prominent acute inflammatory reaction that lead to further tissue damage and injury. An effort is put forth in the present study to develop a bio conjugated selenium nanoparticles (SeNPs) for wound healing which is delayed by infectious microbes. Sericin and essential oil obtained from waste cocoons and W. tinctoria bark was utilized for the synthesis of selenium nanoparticle. The structure of Sr-Wt-SeNPs was characterized by UV-Visible spectroscopy, Fourier Transform Infrared Spectroscopy and X-ray Diffraction, later investigated for their antioxidant, antidiabetic, antithrombotic, antimicrobial and anti-inflammatory efficacy. The spectral studies showed that the Sr-Wt-SeNPs were formed optimally exhibiting a maximum absorption peak at 266 nm. Sr-Wt-SeNPs showed potent IC50 of 34.54 μg/ml and 41.04 μg/ml against DPPH and reducing power assay. The in vitro α-amylase and α-glucosidase inhibitory activity of Sr-Wt-SeNPs was 71% & 66% respectively. The antithrombotic activity was also found to be 50.76%. Similarly, the Sr-Wt-SeNPs (100 μg/mL) showed strong antimicrobial property against the selected gram-positive, gram-negative and fungal pathogens. Anti-inflammatory activity with maximum inhibition value for albumin denaturation and membrane stabilization test (100 μg/ml) for Sr-Wt-SeNPs was potent when compared to individual sericin and essential oil. The present study is an attempt to explore the utility and value of Sericin, a waste material as green material and combination of it with essential oil from W. tinctoria bark to synthesize pharmacologically active SeNPs.

Keywords

Wrightia tinctoria, Sericin, Essential oil, Sr-Wt-SeNPs, antioxidant, anti-diabetic, anti-thrombotic, anti-inflammatory activity

Conclusion

Silk protein sericin is an emerging substance with vast pharmacological properties. In the present study, the Sr-Wr- SeNPs were synthesized by using sericin and essential oil from W. tinctoria and was characterized The synthesized Sr-Wr- SeNPs showed potent antioxidant, antimicrobial activity, anti-inflammatory, antidiabetic and antithrombotic activities when compared to sericin & essential oil the Sr-Wr- SeNPs. Further work is needed to develop wound healing gauze –robustness and efficiency to make it suitable for commercial production.

References

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How to cite this article

M. Sridevi, R. Siva Renjith, A. Surya and C. Nirmala (2023). Wound Healing Gauze Formulation through Synthesis of Selenium Nanoparticles from Sericin and Wrightia tinctoria – A Green Approach. Biological Forum – An International Journal, 15(5): 1103-1114.