Wound Healing Potential of Bioactive Compound from Cayratia trifolia (L.): An In silico and In Vitro analysis

Author: Bhuvaneswari Meganathan and Mani Panagal

Download PDF

Abstract

The process of wound healing involves replacing damaged cellular structures with functional ones. Wounds have been treated with medicinal plants and their active compounds for thousands of years. Chinese and Indian traditional medicines have proven effective in healing. Medicinal plant of Cayratia trifolia L (C. trifolia) has variety of phytocompounds and they extracts possess antiviral, antibacterial, antiprotozoal, hypoglycemic, anticancer, and wound healing properties. Therefore, in this study ethanolic extract of C. trifolia was investigated to determine its bioactivity, structure, and wound healing capability of bioactive compound. Initially, linolenyl alcohol (cmd-1) was identified by chromatographic and spectroscopic methods. Even at highest concentrations of 200 µg/ml in normal fibroblasts cell lines of Normal Human Dermal Fibroblasts cells (NHDF) and Human Umbilical Vein Endothelial cells (HUVEC), it does not appear to be cytotoxic. Molecular docking studies revealed that, when compared with reference drug, cmd-1 have strong binding affinity with wound healing related target proteins such as PKC βII (Protein kinase C βII), TNF-α (Tumor necrosis factor alpha), IL-1β (Interleukin-1 beta), PDGFRA (Platelet-derived growth factor receptor alpha), VEGF-A (Vascular endothelial growth factor A) and TGFBR1 (Transforming Growth Factor Beta Receptor 1) and docking score ranges from -3.8 to -7.1 Kcal/mol. These compounds exhibited acceptable ADME properties. As a result of this study, it can be concluded that the isolated bioactive compound of linolenyl alcohol may be able to heal wounds. Experiments in vitro and in vivo are needed to confirm these findings.

Keywords

Cayratia trifolia L., linolenyl alcohol, Molecular docking, Wound healing

Conclusion

Linolenyl alcohol was isolated and identified by chromatographic and spectroscopic methods from ethanolic extracts of C. trifolia. NHDF and HUVEC fibroblast cell lines were not cytotoxic by cmd-1 at highest concentrations of 200 µg/mL in in vitro cytotoxicity analysis. A computational docking study associated with cmd-1 also observed that it was able to interact well with wound healing targets PDGFRA, VEGF-A, and TGFBR1 kinase with docking scores ranging from -3.8 to -7.1 Kcal/mol. Those compounds showed acceptable ADME properties. This study concludes that linolenyl alcohol isolated from ethanolic extract of C. trifolia may have wound healing properties based on its in vitro and in silico results. For the current funding to be confirmed, further analysis of in vitro and in vivo experimental animal models is needed.

References

-

How to cite this article

Bhuvaneswari Meganathan and Mani Panagal (2023). Wound Healing Potential of Bioactive Compound from Cayratia trifolia (L.): An in silico and in vitro analysis. Biological Forum – An International Journal, 15(5): 104-113.